一种改进的尿液中2,5 -己二酮分析方法。
An improved method of analysing 2,5-hexanedione in urine.
作者信息
Perbellini L, Marhuenda Amoros D M, Cardona Llorens A C, Giuliari C, Brugnone F
机构信息
Institute of Occupation Medicine, University of Verona, Policlinico Borgo Roma, Italy.
出版信息
Br J Ind Med. 1990 Jun;47(6):421-4. doi: 10.1136/oem.47.6.421.
Abstract
A short gas-chromatographic method for analysing urinary concentrations of 2,5-hexanedione is based on acid hydrolysis of urine at pH below 0.1 and "purification" of the urine samples by microcolumns containing an octadecyl-silane phase. A 5% acetonitrile solution allows a fairly selective elution of 2,5-hexanedione from the microcolumns. Recovery of 2,5-hexanedione from urine is as great as 79.9%. The variation coefficient of the measurements is 2.8%. The results obtained from different working conditions and using packed or wide bore or capillary gas-chromatographic columns are reported.
摘要
一种用于分析尿中2,5 - 己二酮浓度的短程气相色谱法，其基于在pH值低于0.1的条件下对尿液进行酸水解，并通过含有十八烷基硅烷相的微柱对尿液样本进行“纯化”。5%的乙腈溶液可使2,5 - 己二酮从微柱中得到相当选择性的洗脱。尿中2,5 - 己二酮的回收率高达79.9%。测量的变异系数为2.8%。报告了在不同工作条件下以及使用填充柱、宽口径柱或毛细管气相色谱柱所获得的结果。
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本文引用的文献
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Br J Ind Med. 1981 Feb;38(1):20-6. doi: 10.1136/oem.38.1.20.
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Measurement of the urinary metabolites of N-hexane, cyclohexane and their isomers by gas chromatography.采用气相色谱法测定正己烷、环己烷及其异构体的尿代谢物。
Int Arch Occup Environ Health. 1981;48(1):99-106. doi: 10.1007/BF00405936.
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A study on biological monitoring of n-hexane exposure.一项关于正己烷暴露生物监测的研究。
Int Arch Occup Environ Health. 1983;51(3):253-60. doi: 10.1007/BF00377756.
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Med Lav. 1985 Jan-Feb;76(1):35-43.
6
Methodological investigations on the determination of n-hexane metabolites in urine.尿中n - 己烷代谢物测定的方法学研究
Int Arch Occup Environ Health. 1986;57(2):149-58. doi: 10.1007/BF00381383.
7
Detection of 2,5-hexanedione in the urine of persons not exposed to n-hexane.未接触正己烷人员尿液中2,5 -己二酮的检测
Int Arch Occup Environ Health. 1986;57(2):143-8. doi: 10.1007/BF00381382.
8
[Gas chromatographic determination of 2,5-hexanedione in the urine].[气相色谱法测定尿液中的2,5 - 己二酮]
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9
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