通过免疫电子显微镜对小圆形结构病毒进行抗原特性分析。
Antigenic characterization of small, round-structured viruses by immune electron microscopy.
作者信息
Okada S, Sekine S, Ando T, Hayashi Y, Murao M, Yabuuchi K, Miki T, Ohashi M
机构信息
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
出版信息
J Clin Microbiol. 1990 Jun;28(6):1244-8. doi: 10.1128/jcm.28.6.1244-1248.1990.
Small, round-structured viruses (SRSVs) detected from nonbacterial gastroenteritis outbreaks in Tokyo and Saitama Prefecture, Japan, during the period from 1977 to 1988 were tentatively classified into nine antigenic patterns from SRSV-1 (S-1) to SRSV-9 (S-9) by cross-immune electron microscopy (IEM). S-1 and S-2 appeared pattern specific, while S-3 to S-9, distinguishable from each other in their reactivity, appeared somewhat antigenically related. Their antigenic relatedness to the Norwal, Hawaii, and Otofuke agents was also examined by IEM by using antisera to these agents. S-3 appeared most closely related to the Norwalk agent. S-4 and S-5 were related to the Norwalk agent and, presumably, were distantly related to the Hawaii and Otofuke agents. S-6 and S-7 were related to the Hawaii and Otofuke agents. S-8 and S-9 were related to the Otofuke agent and, presumably, were distantly related to the Hawaii agent. The prevalence of each antigenic pattern in 38 outbreaks was examined: S-8 was implicated in 24% of the outbreaks S-5 in 16%, S-4 in 13%, S-9 in 13%, S-6 in 11%, and others in 5%.
1977年至1988年期间，在日本东京和埼玉县非细菌性肠胃炎暴发中检测到的小型圆形结构病毒（SRSV），通过交叉免疫电子显微镜（IEM）初步分为9种抗原型，即从SRSV-1（S-1）到SRSV-9（S-9）。S-1和S-2呈现出型特异性，而S-3至S-9虽然在反应性上彼此有区别，但在抗原性上似乎有些关联。还通过IEM使用针对诺沃克、夏威夷和大府 agents 的抗血清来检测它们与这些病原体的抗原相关性。S-3似乎与诺沃克病原体关系最为密切。S-4和S-5与诺沃克病原体有关，并且推测与夏威夷和大府 agents 关系较远。S-6和S-7与夏威夷和大府 agents 有关。S-8和S-9与大府病原体有关，并且推测与夏威夷病原体关系较远。研究了38次暴发中每种抗原型的流行情况：S-8在24%的暴发中出现，S-5在16%中出现，S-4在13%中出现，S-9在13%中出现，S-6在11%中出现，其他在5%中出现。
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