Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, China.
J Dent Res. 2013 Oct;92(10):932-8. doi: 10.1177/0022034513502053. Epub 2013 Aug 19.
The objectives of this study were to synthesize new quaternary ammonium methacrylates (QAMs) with systematically varied alkyl chain lengths (CL) and to investigate, for the first time, the CL effects on antibacterial efficacy, cytotoxicity, and dentin bond strength of bonding agents. QAMs were synthesized with CL of 3 to 18 and incorporated into Scotchbond Multi-Purpose (SBMP) bonding agent. The cured resins were inoculated with Streptococcus mutans. Bacterial early attachment was investigated at 4 hrs. Biofilm colony-forming units (CFU) were measured after 2 days. With CL increasing from 3 to 16, the minimum inhibitory concentration and minimum bactericidal concentration were decreased by 5 orders of magnitude. Incorporating QAMs into SBMP reduced bacterial early attachment, with the least colonization at CL = 16. Biofilm CFU for CL = 16 was 4 log lower than SBMP control (p < .05). All groups had similar dentin bond strengths (p > .1). The new antibacterial materials had fibroblast/odontoblast viability similar to that of commercial controls. In conclusion, increasing the chain length of new QAMs in bonding agents greatly increased the antibacterial efficacy. A reduction in Streptococcus mutans biofilm CFU by 4 log could be achieved, without compromising bond strength and cytotoxicity. New QAM-containing bonding agents are promising for a wide range of restorations to inhibit biofilms.
本研究的目的是合成具有系统变化的烷基链长度（CL）的新型季铵甲基丙烯酸酯（QAMs），并首次研究 CL 对粘结剂的抗菌功效、细胞毒性和牙本质粘结强度的影响。合成了 CL 为 3 至 18 的 QAMs，并将其掺入 Scotchbond Multi-Purpose（SBMP）粘结剂中。将固化树脂接种变形链球菌。在 4 小时时研究细菌早期附着。2 天后测量生物膜集落形成单位（CFU）。随着 CL 从 3 增加到 16，最低抑菌浓度和最低杀菌浓度降低了 5 个数量级。将 QAMs 掺入 SBMP 中减少了细菌的早期附着，CL = 16 时的定植最少。CL = 16 的生物膜 CFU 比 SBMP 对照组低 4 个对数级（p <.05）。所有组的牙本质粘结强度相似（p >.1）。新型抗菌材料的成纤维细胞/成牙本质细胞活力与商业对照相似。总之，在粘结剂中增加新型 QAMs 的链长大大提高了抗菌功效。通过减少 4 个对数级的变形链球菌生物膜 CFU，可以在不损害粘结强度和细胞毒性的情况下实现。新型含 QAM 的粘结剂有望广泛用于各种修复以抑制生物膜。
