Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Avenida Colombo 5.790, 87020-900 Maringá, PR, Brazil.
Evid Based Complement Alternat Med. 2013;2013:795916. doi: 10.1155/2013/795916. Epub 2013 Jul 21.
Naringenin and quercetin are considered antioxidant compounds with promising activity against oxidative damage in human cells. However, no reports have described their effects on reactive oxygen species (ROS) production by phagocytes during microbicidal activity. Thus, the present study evaluated the effects of naringenin and quercetin on ROS production, specifically hypochlorous acid (HOCl), and their involvement in the microbicidal activity of neutrophils. Naringenin and quercetin inhibited HOCl production through different systems, but this inhibition was more pronounced for quercetin, even in the cell-free systems. With regard to the microbicidal activity of neutrophils, both naringenin and quercetin completely inhibited the killing of Staphylococcus aureus. Altogether, these data indicate that the decrease in the oxidant activity of neutrophils induced by these compounds directly impaired the microbicidal activity of neutrophils. Naringenin and quercetin exerted their effects by controlling the effector mechanisms of ROS production, with both positive and negative effects of these antioxidant agents in oxidative stress conditions and on ROS in the microbicidal activity of phagocytes. The present results challenge the traditional view of antioxidants as improvers of pathological conditions.
柚皮素和槲皮素被认为是具有抗氧化活性的化合物，对人类细胞的氧化损伤具有潜在的作用。然而，目前尚无报道描述它们在吞噬细胞杀菌活性过程中对活性氧（ROS）产生的影响。因此，本研究评估了柚皮素和槲皮素对 ROS 产生（特别是次氯酸（HOCl））的影响，及其在中性粒细胞杀菌活性中的作用。柚皮素和槲皮素通过不同的系统抑制 HOCl 的产生，但槲皮素的抑制作用更为明显，即使在无细胞系统中也是如此。关于中性粒细胞的杀菌活性，柚皮素和槲皮素均完全抑制了金黄色葡萄球菌的杀伤作用。综上所述，这些数据表明，这些化合物诱导的中性粒细胞氧化剂活性的降低直接损害了中性粒细胞的杀菌活性。柚皮素和槲皮素通过控制 ROS 产生的效应机制发挥作用，这些抗氧化剂在氧化应激条件下和吞噬细胞的杀菌活性中的 ROS 具有正反两方面的作用。本研究结果对传统的抗氧化剂作为改善病理状况的观点提出了挑战。
