Gdansk University of Physical Education and Sport, Department of Physiology , Poland;
J Sports Sci Med. 2013 Jun 1;12(2):282-9. eCollection 2013.
Heat shock proteins (Hsp) represent proteins' groups, whose protective function, may be induced by heat, reactive oxygen species, cytokines etc. We evaluated blood levels of Hsp27 and Hsp70, and their relation to skeletal muscle damage and inflammation in young tennis players before and after the conditioning camp. Blood samples were collected directly after tournament season, 3-day rest and 14-day conditioning camp that followed. Hydrogen peroxide (H2O2) demonstrated the highest concentration directly after tournament season, which significantly decreased at camp's end. The pro-inflammatory cytokines IL-1β and TNFα decreased, whereas anti-inflammatory cytokines IL-6 and IL-10 increased after 3d rest and 14d camp. Hsp27 increased after 3d rest and remained so after 14d camp, while Hsp70 decreased from baseline to camp's completion. Hsp27 and Hsp70 correlated significantly with H2O2, IL-1β and TNFα. Muscle damage, observed as creatine kinase (CK) activity changes, increased after 14d camp similarly to Hsp27 and anti-inflammatory cytokines IL-6 and IL-10. Obtained data allows to conclude that decrease of Hsp27 and increase in pro-inflammatory cytokines could be a good indicator of overreaching. Reverse tendencies in these proteins may verify accuracy of conditioning camp. Finally, this training program caused an increase in the anti-inflammatory cytokines concentrations, improving individual status of recovery. Key PointsThe study demonstrating low grade inflammation-induced by the tournament season in young tennis player.Three days of active rest stimulated the anti-inflammatory response via rise of Hsp27 and anti-inflammatory cytokine IL-10.Observed decrease of blood Hsp70 may support mental recovery.Thirteen-day appropriate training program led to maintaining an immunological response balance.
热休克蛋白（Hsp）代表蛋白质的一个家族，其保护功能可能是由热、活性氧物质、细胞因子等诱导的。我们评估了 HSP27 和 HSP70 的血液水平，以及它们与年轻网球运动员在调整训练营前后骨骼肌损伤和炎症的关系。血液样本在赛季结束后、3 天休息后和随后的 14 天调整训练营中直接采集。过氧化氢（H2O2）在赛季结束后表现出最高浓度，在训练营结束时显著降低。促炎细胞因子 IL-1β 和 TNFα 减少，而抗炎细胞因子 IL-6 和 IL-10 在 3 天休息和 14 天训练营后增加。Hsp27 在 3 天休息后增加，并在 14 天训练营后保持不变，而 Hsp70 从基线下降到训练营结束。Hsp27 和 Hsp70 与 H2O2、IL-1β 和 TNFα 呈显著相关。肌酸激酶（CK）活性变化观察到的肌肉损伤在 14 天训练营后与 Hsp27 和抗炎细胞因子 IL-6 和 IL-10 相似增加。获得的数据允许得出结论，Hsp27 的减少和促炎细胞因子的增加可能是过度训练的良好指标。这些蛋白质的反向趋势可能验证调整训练营的准确性。最后，这个训练计划导致抗炎细胞因子浓度的增加，改善了个体的恢复状态。关键点本研究证明了年轻网球运动员在比赛季节中存在低度炎症。三天的主动休息通过增加 HSP27 和抗炎细胞因子 IL-10 刺激抗炎反应。观察到血液 HSP70 的减少可能支持心理恢复。为期 13 天的适当训练计划导致免疫反应平衡得以维持。
