Lai Charles P, Tannous Bakhos A, Breakefield Xandra O
Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.
Methods Mol Biol. 2014;1098:249-58. doi: 10.1007/978-1-62703-718-1_19.
Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo.
细胞外囊泡（EVs），包括外泌体和微囊泡，是细胞释放的纳米级囊泡，用于递送脂质、细胞蛋白、信使核糖核酸（mRNAs）和非编码核糖核酸（RNAs），从而在细胞间无需直接接触的情况下促进细胞间通讯。由于其纳米级尺寸，EVs已在体外显微镜下可视化。我们在此描述一种用高斯荧光素酶标记EVs的策略，用于体内无创生物发光成像和监测全身给药的EVs。
