大肠杆菌敲除株的代谢通量分析：来自 Keio 文库的经验教训和未来展望。
Metabolic flux analysis of Escherichia coli knockouts: lessons from the Keio collection and future outlook.
机构信息
Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, Newark, DE 19716, USA.
Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, Newark, DE 19716, USA.
出版信息
Curr Opin Biotechnol. 2014 Aug;28:127-33. doi: 10.1016/j.copbio.2014.02.006. Epub 2014 Mar 28.
Abstract
Cellular metabolic and regulatory systems are of fundamental interest to biologists and engineers. Incomplete understanding of these complex systems remains an obstacle to progress in biotechnology and metabolic engineering. An established method for obtaining new information on network structure, regulation and dynamics is to study the cellular system following a perturbation such as a genetic knockout. The Keio collection of all viable Escherichia coli single-gene knockouts is facilitating a systematic investigation of the regulation and metabolism of E. coli. Of all omics measurements available, the metabolic flux profile (the fluxome) provides the most direct and relevant representation of the cellular phenotype. Recent advances in (13)C-metabolic flux analysis are now permitting highly precise and accurate flux measurements for investigating cellular systems and guiding metabolic engineering efforts.
摘要
细胞代谢和调节系统是生物学家和工程师非常感兴趣的基础。对这些复杂系统的不完全了解仍然是生物技术和代谢工程进展的障碍。获得有关网络结构、调节和动力学的新信息的一种既定方法是在受到诸如基因敲除等扰动后研究细胞系统。京都大肠杆菌单基因敲除菌集合正在促进对大肠杆菌的调节和代谢的系统研究。在所有可用的组学测量中，代谢通量谱（通量组）为细胞表型提供了最直接和最相关的表示。（13）C 代谢通量分析的最新进展现在允许进行高度精确和准确的通量测量，以研究细胞系统并指导代谢工程工作。
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