Jackson Emily L, Lu Hang
School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Dr. NW, Atlanta, GA 30332-0100, USA.
Curr Opin Chem Eng. 2013 Nov 1;2(4):398-404. doi: 10.1016/j.coche.2013.10.001.
Cellular separations are required in many contexts in biochemical and biomedical applications for the identification, isolation, and analysis of phenotypes or samples of interest. Microfluidics is uniquely suited for handling biological samples, and emerging technologies have become increasingly accessible tools for researchers and clinicians. Here, we review advances in the last few years in techniques for microfluidic cell separation and manipulation. Applications such as high-throughput cell and organism phenotypic screening, purification of heterogeneous stem cell populations, separation of blood components, and isolation of rare cells in patients highlight some of the areas in which these technologies show great potential. Continued advances in separation mechanisms and understanding of cellular systems will yield further improvements in the throughput, resolution, and robustness of techniques.
在生化和生物医学应用的许多情况下，为了识别、分离和分析感兴趣的表型或样本，都需要进行细胞分离。微流体技术特别适合处理生物样本，并且新兴技术已成为研究人员和临床医生越来越容易获得的工具。在这里，我们回顾了过去几年微流体细胞分离和操作技术的进展。诸如高通量细胞和生物体表型筛选、异质干细胞群体的纯化、血液成分的分离以及患者体内稀有细胞的分离等应用突出了这些技术具有巨大潜力的一些领域。分离机制和对细胞系统理解的持续进展将进一步提高技术的通量、分辨率和稳健性。
