Charoenphol Phapanin, Bermudez Harry
Department of Polymer Science and Engineering, University of Massachusetts , Amherst, Massachusetts 01003, United States.
Mol Pharm. 2014 May 5;11(5):1721-5. doi: 10.1021/mp500047b. Epub 2014 Apr 17.
DNA-based nanostructures have been widely used in various applications due to their structural diversity, programmability, and uniform structures. Their intrinsic biocompatibility and biodegradability further motivates the investigation of DNA-based nanostructures as delivery vehicles. Incorporating AS1411 aptamers into DNA pyramids leads to enhanced intracellular uptake and selectively inhibits the growth of cancer cells, achieved without the use of transfection reagents. Furthermore, aptamer-displaying pyramids are found to be substantially more resistant to nuclease degradation than single-stranded aptamers. These findings, along with their modularity, reinforce the potential of DNA-based nanostructures for therapeutic applications.
基于DNA的纳米结构因其结构多样性、可编程性和均匀结构，已被广泛应用于各种领域。它们固有的生物相容性和生物可降解性进一步推动了对基于DNA的纳米结构作为递送载体的研究。将AS1411适配体整合到DNA金字塔中可增强细胞内摄取，并选择性抑制癌细胞生长，且无需使用转染试剂即可实现。此外，发现展示适配体的金字塔比单链适配体对核酸酶降解的抵抗力要强得多。这些发现及其模块化特性，增强了基于DNA的纳米结构在治疗应用中的潜力。
