Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong.
Evid Based Complement Alternat Med. 2014;2014:608721. doi: 10.1155/2014/608721. Epub 2014 Mar 16.
Chemical change during boiling of herbal mixture is a puzzle. By using Danggui Buxue Tang (DBT), a herbal decoction that contains Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), we developed a model in analyzing the hydrolysis of flavonoid glycosides during the boiling of herbal mixture in water. A proper preparation of DBT is of great benefit to the complete extraction of bioactive ingredients. Boiling of DBT in water increased the solubility of AR-derived astragaloside IV, calycosin, formononetin, calycosin-7-O- β -D-glucoside, and ononin in a time- and temperature-dependent manner: the amounts of these chemicals reached a peak at 2 h. The glycosidic resides of AR, calycosin-7-O- β -D-glucoside, and ononin could be hydrolyzed during the moderate boiling process to form calycosin and formononetin, respectively. The hydrolysis efficiency was strongly affected by pH, temperature, and amount of herbs. Interestingly, the preheated herbs were not able to show this hydrolytic activity. The current results supported the rationality of ancient preparation of DBT in boiling water by moderate heat.
草药混合物在煮沸过程中的化学变化是一个谜。通过使用当归补血汤（DBT），一种含有黄芪（AR）和当归（ASR）的草药煎剂，我们开发了一种模型来分析在水中煮沸草药混合物时黄酮苷的水解。DBT 的适当制备对于完全提取生物活性成分非常有益。在水中煮沸 DBT 会增加 AR 衍生的黄芪甲苷 IV、毛蕊异黄酮、芒柄花素、毛蕊异黄酮-7-O-β-D-葡萄糖苷和芒柄花苷在时间和温度依赖性方式下的溶解度：这些化学物质的量在 2 小时时达到峰值。AR、毛蕊异黄酮-7-O-β-D-葡萄糖苷和芒柄花苷的糖苷残基可以在适度的煮沸过程中水解，分别形成毛蕊异黄酮和芒柄花素。水解效率强烈受 pH 值、温度和草药用量的影响。有趣的是，预热的草药没有表现出这种水解活性。目前的结果支持了古代在热水中用适度热量制备 DBT 的合理性。
