来自尖叶山蚂蝗的异黄酮及其细胞毒性。

Isoflavanones from Desmodium oxyphyllum and their cytotoxicity.

作者信息

Li Yan-Ping, Li Yin-Ke, Du Gang, Yang Hai-Yin, Gao Xue-Mei, Hu Qiu-Fen

机构信息

a School of Pharmacy, Yunnan University of Traditional Chinese Medicine , Kunming 650500 , China.

出版信息

J Asian Nat Prod Res. 2014;16(7):735-40. doi: 10.1080/10286020.2014.906406. Epub 2014 Apr 22.

DOI:10.1080/10286020.2014.906406

PMID:24749537

Abstract

Two new isoflavanones, (3R)-7-hydroxy-4'-methoxy-5-methoxycarbonyl-isoflavanone (1) and (3R)-8-hydroxy-4'-methoxy-7-methoxycarbonyl-isoflavanone (2), together with seven known isoflavanones (3-9) were isolated from Desmodium oxyphyllum of the Leguminosae family. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Compound 1 showed good cytotoxicity against NB4 and SHSY5Y cell lines with IC50 values of 3.1 and 2.5 μM; compound 2 exhibited cytotoxicity against PC3 cell lines with a IC50 value of 3.6 μM; compound 4 showed cytotoxicity against A549 and SHSY5Y cell lines with IC50 values of 3.6 and 2.8 μM; and compound 5 displayed cytotoxicity against NB4, SHSY5Y, and MCF7 cell lines with IC50 values of 2.6, 3.8, and 2.8 μM, respectively. Other compounds also showed moderate cytotoxicity for some tested cell lines with IC50 values between 5.4 and 8.8 μM.

摘要

从豆科植物尖叶山蚂蝗中分离得到两个新的异黄酮类化合物，(3R)-7-羟基-4'-甲氧基-5-甲氧基羰基异黄酮（1）和(3R)-8-羟基-4'-甲氧基-7-甲氧基羰基异黄酮（2），以及七个已知的异黄酮类化合物（3 - 9）。通过光谱方法，包括广泛的一维和二维核磁共振技术对它们的结构进行了阐明。化合物1对NB4和SHSY5Y细胞系显示出良好的细胞毒性，IC50值分别为3.1和2.5 μM；化合物2对PC3细胞系表现出细胞毒性，IC50值为3.6 μM；化合物4对A549和SHSY5Y细胞系显示出细胞毒性，IC50值分别为3.6和2.8 μM；化合物5对NB4、SHSY5Y和MCF7细胞系表现出细胞毒性，IC50值分别为2.6、3.8和2.8 μM。其他化合物对一些测试细胞系也显示出中等细胞毒性，IC50值在5.4至8.8 μM之间。