Hirschi Karen K, Li Song, Roy Krishnendu
Yale Cardiovascular Research Center and Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut 06511; email:
Annu Rev Biomed Eng. 2014 Jul 11;16:277-94. doi: 10.1146/annurev-bioeng-071813-105108. Epub 2014 May 29.
With the discovery of induced pluripotent stem (iPS) cells, it is now possible to convert differentiated somatic cells into multipotent stem cells that have the capacity to generate all cell types of adult tissues. Thus, there is a wide variety of applications for this technology, including regenerative medicine, in vitro disease modeling, and drug screening/discovery. Although biological and biochemical techniques have been well established for cell reprogramming, bioengineering technologies offer novel tools for the reprogramming, expansion, isolation, and differentiation of iPS cells. In this article, we review these bioengineering approaches for the derivation and manipulation of iPS cells and focus on their relevance to regenerative medicine.
随着诱导多能干细胞（iPS细胞）的发现，现在有可能将分化的体细胞转化为具有生成成人组织所有细胞类型能力的多能干细胞。因此，这项技术有广泛的应用，包括再生医学、体外疾病建模以及药物筛选/发现。尽管用于细胞重编程的生物学和生物化学技术已经很成熟，但生物工程技术为iPS细胞的重编程、扩增、分离和分化提供了新工具。在本文中，我们综述了这些用于iPS细胞衍生和操作的生物工程方法，并重点关注它们与再生医学的相关性。
