Abbate V, Kicman A T, Evans-Brown M, McVeigh J, Cowan D A, Wilson C, Coles S J, Walker C J
Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK.
Drug Control Centre, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK.
Drug Test Anal. 2015 Jul;7(7):609-18. doi: 10.1002/dta.1728. Epub 2014 Oct 6.
Twenty-four products suspected of containing anabolic steroids and sold in fitness equipment shops in the United Kingdom (UK) were analyzed for their qualitative and semi-quantitative content using full scan gas chromatography-mass spectrometry (GC-MS), accurate mass liquid chromatography-mass spectrometry (LC-MS), high pressure liquid chromatography with diode array detection (HPLC-DAD), UV-Vis, and nuclear magnetic resonance (NMR) spectroscopy. In addition, X-ray crystallography enabled the identification of one of the compounds, where reference standard was not available. Of the 24 products tested, 23 contained steroids including known anabolic agents; 16 of these contained steroids that were different to those indicated on the packaging and one product contained no steroid at all. Overall, 13 different steroids were identified; 12 of these are controlled in the UK under the Misuse of Drugs Act 1971. Several of the products contained steroids that may be considered to have considerable pharmacological activity, based on their chemical structures and the amounts present. This could unwittingly expose users to a significant risk to their health, which is of particular concern for naïve users.
利用全扫描气相色谱-质谱联用仪（GC-MS）、精确质量液相色谱-质谱联用仪（LC-MS）、带二极管阵列检测的高压液相色谱仪（HPLC-DAD）、紫外可见分光光度计和核磁共振波谱仪（NMR），对在英国健身器材商店销售的24种疑似含有合成代谢类固醇的产品进行了定性和半定量分析。此外，在没有参考标准品的情况下，X射线晶体学技术成功鉴定出了其中一种化合物。在测试的24种产品中，有23种含有类固醇，包括已知的合成代谢剂；其中16种所含的类固醇与包装上标明的不同，还有一种产品根本不含类固醇。总体而言，共鉴定出13种不同的类固醇；其中12种在英国受1971年《滥用药物法》管制。基于这些产品中类固醇的化学结构和含量，其中几种可能具有相当大的药理活性。这可能会在不知不觉中将使用者置于重大健康风险之下，这对缺乏经验的使用者来说尤其令人担忧。
