Behbahani Mandana
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Islamic Republic of Iran.
PLoS One. 2014 Oct 21;9(10):e108195. doi: 10.1371/journal.pone.0108195. eCollection 2014.
Monofloral Iranian honeys from eight floral sources were analyzed to determine their anti-HIV-1 activities as well as their effects on lymphocyte proliferation. The Peripheral Blood Mononuclear Cells (PBMCs) used in this study were prepared from five healthy volunteers who were seronegative for HIV, HCV, HBV and TB. The anti-HIV-1 activity of eight different honeys was performed by quantitative polymerase chain reaction (PCR) assay and high pure viral nucleic acid kit. The results demonstrated that monofloral honeys from Petro selinum sativum, Nigella sativa, Citrus sinensis, Zataria multiflora, Citrus aurantium and Zizyphus mauritiana flowers had potent anti-HIV-1 activity with half maximal effective concentration (EC50) values of 37.5, 88, 70, 88, 105 and 5 µg/ml respectively. However, monofloral Iranian honeys from Astragalus gummifer and Chamaemelum nobile flowers had weak anti-HIV-1 activity. The frequency and intensity of CD4 expression on PBMCs increased in the presence of all honey types. CD19 marker were also increased after the treatment with monofloral honeys from Z. multiflora and N. sativa. The anti-HIV-1 agent in monofloral honeys from P. sativum, N. sativa, Z. multiflora and Z. mauritiana flowers was detected by spectroscopic analysis as methylglyoxal. Time of drug addition studies demonstrated that the inhibitory effect of methylglyoxal is higher on the late stage of HIV-1 infection. The result demonstrated that methylglyoxal isolated from monofloral honey types is a good candidate for preclinical evaluation of anti-HIV-1 therapies.
对来自八个花源的伊朗单花蜂蜜进行了分析，以确定它们的抗HIV-1活性以及对淋巴细胞增殖的影响。本研究中使用的外周血单个核细胞（PBMC）取自五名HIV、HCV、HBV和TB血清学阴性的健康志愿者。通过定量聚合酶链反应（PCR）测定和高纯病毒核酸试剂盒检测了八种不同蜂蜜的抗HIV-1活性。结果表明，来自欧芹、黑种草、甜橙、多花百里香、酸橙和毛里求斯枣花的单花蜂蜜具有较强的抗HIV-1活性，半数最大有效浓度（EC50）值分别为37.5、88、70、88、105和5μg/ml。然而，来自胶黄芪和果香菊花朵的伊朗单花蜂蜜的抗HIV-1活性较弱。在所有类型蜂蜜存在的情况下，PBMC上CD4表达的频率和强度增加。用多花百里香和黑种草的单花蜂蜜处理后，CD19标志物也增加。通过光谱分析检测到，来自欧芹、黑种草、多花百里香和毛里求斯枣花的单花蜂蜜中的抗HIV-1剂为甲基乙二醛。药物添加时间研究表明，甲基乙二醛对HIV-1感染后期的抑制作用更高。结果表明，从单花蜂蜜类型中分离出的甲基乙二醛是抗HIV-1疗法临床前评估的良好候选物。
