Skolnik P R, Pomerantz R J, de la Monte S M, Lee S F, Hsiung G D, Foos R Y, Cowan G M, Kosloff B R, Hirsch M S, Pepose J S
Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts.
Am J Ophthalmol. 1989 Apr 15;107(4):361-72. doi: 10.1016/0002-9394(89)90659-4.
We examined retinal tissue from eight human immunodeficiency virus type 1 (HIV-1) seropositive patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex for evidence of dual infection with HIV-1 and cytomegalovirus. Culture demonstrated simultaneous infection with HIV-1 and cytomegalovirus in two of 13 retinal specimens. This was confirmed by both immunofluorescence and immunohistochemical staining. Moreover, coinfection of individual cells with cytomegalovirus and HIV-1 was observed by immunohistochemical staining. Infection of retina with cytomegalovirus or HIV-1 alone occurred in one and six of the 13 retinal specimens, respectively. HIV-1 antigens were present on scattered cells in all layers of the retina and on retinal vascular endothelium. HIV-1 was isolated from retinal tissue derived from eyes both with and without gross ocular lesions. Cytomegalovirus antigens were found in all layers of the retina, but not on vascular endothelial cells. The atypically rapid clinical progression of retinitis in one of the patients with dual HIV-1 and cytomegalovirus infection suggests the possibility that interactions between these two viruses may influence retinal disease in patients with AIDS.
我们检查了8名1型人类免疫缺陷病毒（HIV-1）血清反应阳性且患有获得性免疫缺陷综合征（AIDS）或AIDS相关综合征患者的视网膜组织，以寻找HIV-1和巨细胞病毒双重感染的证据。培养显示，在13份视网膜标本中有2份同时感染了HIV-1和巨细胞病毒。免疫荧光和免疫组织化学染色均证实了这一点。此外，通过免疫组织化学染色观察到个别细胞同时感染了巨细胞病毒和HIV-1。在13份视网膜标本中，分别有1份和6份仅感染了巨细胞病毒或HIV-1。HIV-1抗原存在于视网膜各层的散在细胞以及视网膜血管内皮细胞上。从有和没有明显眼部病变的眼睛获取的视网膜组织中均分离出了HIV-1。在视网膜各层均发现了巨细胞病毒抗原，但在血管内皮细胞上未发现。1例同时感染HIV-1和巨细胞病毒的患者视网膜病变出现异常快速的临床进展，这表明这两种病毒之间的相互作用可能影响AIDS患者的视网膜疾病。
