Qavi H B, Green M T, SeGall G K, Font R L
Division of Molecular Virology, Baylor College of Medicine, Houston, TX 77030.
Curr Eye Res. 1989 Apr;8(4):379-87. doi: 10.3109/02713688908996385.
Using an immunohistochemical technique and monoclonal antisera, HIV-1 and CMV antigens were demonstrated in lesioned areas of retinal tissues from selected AIDS patients. Polymerase chain reaction (PCR) was utilized to detect HIV-1 and HHV-6 DNA sequences in total retinal tissues from these patients. In this study of six eyes from four patients, two of the retinas contained three different viruses, HIV-1, HHV-6 and CMV. To determine whether HIV-1 and HHV-6 DNA sequences were restricted to the intraretinal lesions, normal and lesioned areas were dissected from the retina, DNA was extracted and subjected to amplification using PCR. The results showed that HIV-1 and HHV-6 DNA were restricted to the lesioned areas. All four lesions (from two different patients) utilized in this study showed the presence of CMV antigens immunohistochemically. The combination of viruses present in each lesion was either HIV-1 and CMV or HHV-6 and CMV. Two of four lesions contained HIV-1 and CMV; a third lesion showed the presence of HHV-6 and CMV. The fourth lesion contained only CMV antigens.
运用免疫组织化学技术和单克隆抗血清，在部分艾滋病患者视网膜组织的病变区域检测到了HIV-1和巨细胞病毒（CMV）抗原。利用聚合酶链反应（PCR）检测这些患者整个视网膜组织中的HIV-1和人疱疹病毒6型（HHV-6）DNA序列。在这项对4名患者的6只眼睛进行的研究中，其中2只视网膜含有三种不同病毒，即HIV-1、HHV-6和CMV。为确定HIV-1和HHV-6 DNA序列是否局限于视网膜内病变区域，从视网膜中分离出正常和病变区域，提取DNA并使用PCR进行扩增。结果显示，HIV-1和HHV-6 DNA局限于病变区域。本研究中使用的所有4个病变组织（来自2名不同患者）经免疫组织化学检测均显示存在CMV抗原。每个病变组织中存在的病毒组合要么是HIV-1和CMV，要么是HHV-6和CMV。4个病变组织中有2个含有HIV-1和CMV；第三个病变组织显示存在HHV-6和CMV。第四个病变组织仅含有CMV抗原。
