Shakoori Zahra, Salimian Samaneh, Kharrazi Sharmin, Adabi Mahdi, Saber Reza
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Anal Bioanal Chem. 2015 Jan;407(2):455-61. doi: 10.1007/s00216-014-8303-9. Epub 2014 Nov 16.
The purpose of this work was to fabricate an electrochemical DNA biosensor for detecting hepatitis B virus. Gold nanorods (GNRs), which are known for their conductivity, were used to increase surface area and consequently increase the immobilization of single-stranded DNA (ss-DNA) on the modified gold electrode. The GNRs were characterized via transmission electron microscopy. The morphology of the gold electrode before and after modification with GNRs was characterized by scanning electron microscopy. Atomic-force microscopy was used to evaluate the morphology of the GNR electrode surface before and after interaction with ss-DNA. Cyclic voltammetry was used to monitor DNA immobilization and hybridization, using Co(phen)3 as an electrochemical indicator. The target DNA sequences were quantified at a linear range from 1.0 × 10(-12) to 10.0 × 10(-6) mol L(-1), with a detection limit of 2.0 × 10(-12) mol L(-1) by 3σ. The biosensor had good specificity for distinguishing complementary DNA in the presence of non-complementary and mismatched DNA sequences.
这项工作的目的是制造一种用于检测乙型肝炎病毒的电化学DNA生物传感器。以其导电性而闻名的金纳米棒（GNRs）被用于增加表面积，从而增加单链DNA（ss-DNA）在修饰金电极上的固定量。通过透射电子显微镜对GNRs进行了表征。用扫描电子显微镜对修饰GNRs前后金电极的形态进行了表征。原子力显微镜用于评估与ss-DNA相互作用前后GNR电极表面的形态。使用Co(phen)3作为电化学指示剂，通过循环伏安法监测DNA的固定和杂交。目标DNA序列在1.0×10(-12)至10.0×10(-6) mol L(-1)的线性范围内进行定量，通过3σ计算检测限为2.0×10(-12) mol L(-1)。该生物传感器在存在非互补和错配DNA序列的情况下，对区分互补DNA具有良好的特异性。
