Wunderli W, Kägi M K, Grüter E, Auracher J D
Institute of Immunology and Virology, University of Zürich, Switzerland.
J Clin Microbiol. 1989 Aug;27(8):1916-7. doi: 10.1128/jcm.27.8.1916-1917.1989.
Screening leukocytes for cytomegalovirus (CMV) by shell vial assay gave unsatisfactory results. Of 10 positive specimens (114 samples), only 2 showing CMV could be detected. Disruption of leukocytes prior to their use in shell vial assays increased the sensitivity of CMV detection considerably: of 32 leukocyte specimens from transplant patients with signs of CMV disease, 13 were positive with disruption and only 3 were positive without. With this modification, 17 transplant patients with suspected CMV infection were regularly screened. Viremia could be detected in 10 cases by the shell vial assay and in 11 cases by the direct detection of immediate early antigen. On the average, viremia was detected 11 days before immunoglobulin M or typical clinical symptoms.
通过空斑试验筛查白细胞中的巨细胞病毒(CMV),结果并不理想。在10个阳性标本(114份样本)中,仅能检测到2份显示CMV阳性。在用于空斑试验之前对白细胞进行破坏,可显著提高CMV检测的灵敏度:在32份来自有CMV疾病体征的移植患者的白细胞标本中,经破坏处理后13份呈阳性,未经处理的仅有3份呈阳性。通过这种改进方法,对17例疑似CMV感染的移植患者进行了定期筛查。通过空斑试验在10例患者中检测到病毒血症,通过直接检测即刻早期抗原则在11例患者中检测到病毒血症。平均而言,在免疫球蛋白M或典型临床症状出现前11天检测到病毒血症。