Department of Food Science and Nutrition, Dankook University, 152 Jukjeon-Ro, Suji-Gu, Yongin-Si, Gyunggi, 448-701, Korea.
Nutr Res Pract. 2015 Feb;9(1):30-6. doi: 10.4162/nrp.2015.9.1.30. Epub 2015 Jan 28.
BACKGROUD/OBEJECTIVES: The mechanism of how black garlic effects lipid metabolism remains unsolved. Therefore, the objectives of this study were to determine the effects of black garlic on lipid profiles and the expression of related genes in rats fed a high fat diet.
MATERIALS/METHODS: Thirty-two male Sqrague-Dawley rats aged 4 weeks were randomly divided into four groups (n=8) and fed the following diets for 5 weeks: normal food diet, (NF); a high-fat diet (HF); and a high-fat diet + 0.5% or 1.5% black garlic extract (HFBG0.5 or HFBG1.5). Body weights and blood biochemical parameters, including lipid profiles, and expressions of genes related to lipid metabolism were determined.
Significant differences were observed in the final weights between the HFBG1.5 and HF groups. All blood biochemical parameters measured in the HFBG1.5 group showed significantly lower values than those in the HF group. Significant improvements of the plasama lipid profiles as well as fecal excretions of total lipids and triglyceride (TG) were also observed in the HFBG1.5 group, when compared to the HF diet group. There were significant differences in the levels of mRNA of sterol regulatory element binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and glucose-6-phosphate dehydrogenase (G6PDH) in the HFBG1.5 group compared to the HF group. In addition, the hepatic expression of (HMG-CoA) reductase and Acyl-CoA cholesterol acyltransferase (ACAT) mRNA was also significantly lower than the HF group.
Consumption of black garlic extract lowers SREBP-1C mRNA expression, which causes downregulation of lipid and cholestrol metahbolism. As a result, the blood levels of total lipids, TG, and cholesterol were decreased.
背景/目的：黑蒜影响脂质代谢的机制尚不清楚。因此，本研究的目的是确定黑蒜提取物对高脂饮食大鼠血脂谱和相关基因表达的影响。
材料/方法：32 只 4 周龄雄性 SD 大鼠随机分为 4 组（n=8），分别用以下饮食喂养 5 周：正常饮食（NF）；高脂肪饮食（HF）；高脂肪饮食+0.5%或 1.5%黑蒜提取物（HFBG0.5 或 HFBG1.5）。测定体重和血液生化参数，包括血脂谱和与脂质代谢相关的基因表达。
HFBG1.5 组和 HF 组的最终体重差异有统计学意义。HFBG1.5 组所有血液生化参数均明显低于 HF 组。与 HF 饮食组相比，HFBG1.5 组还明显改善了血浆脂质谱以及总脂质和甘油三酯（TG）的粪便排泄。与 HF 组相比，HFBG1.5 组固醇调节元件结合蛋白-1c（SREBP-1c）、乙酰辅酶 A 羧化酶（ACC）、脂肪酸合酶（FAS）和葡萄糖-6-磷酸脱氢酶（G6PDH）的 mRNA 水平也有显著差异。此外，HFBG1.5 组肝内（HMG-CoA）还原酶和酰基辅酶 A 胆固醇酰基转移酶（ACAT）mRNA 的表达也明显低于 HF 组。
黑蒜提取物的摄入降低了 SREBP-1C mRNA 的表达，导致脂质和胆固醇代谢下调。因此，总脂质、TG 和胆固醇的血液水平降低。
