Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
Phys Rev Lett. 2015 Mar 6;114(9):098102. doi: 10.1103/PhysRevLett.114.098102. Epub 2015 Mar 2.
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
我们提出了一种基于实验测量的自由电子激光 X 射线衍射线的巨拟病毒颗粒的三维重构的概念验证。三维成像需要将许多二维模式组装成一个内部一致的傅立叶体积。由于每个颗粒在暴露于 X 射线脉冲时都是随机取向的，因此相对取向必须仅从衍射数据中恢复。我们使用一种改进的扩展、最大化和压缩算法来实现这一点，并使用新方法验证我们的结果。
