Eick Sigrun, Ramseier Christoph A, Rothenberger Kathrin, Brägger Urs, Buser Daniel, Salvi Giovanni E
Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Department of Reconstructive Dentistry and Gerodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Clin Oral Implants Res. 2016 Feb;27(2):218-25. doi: 10.1111/clr.12588. Epub 2015 Apr 1.
To determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface.
Plaque samples obtained from the deepest sites of 504 implants and of 493 adjacent teeth were analyzed for certain bacterial species associated with periodontitis, for staphylococci, for aerobic gram-negative rods, and for yeasts using nucleic acid-based methods.
Species known to be associated with periodontitis were detectable at 6.2-78.4% of the implants. Significantly higher counts at implants in comparison with teeth were assessed for Tannerella forsythia, Parvimonas micra, Fusobacterium nucleatum/necrophorum, and Campylobacter rectus. Higher counts of periodontopathogenic species were detectable at implants of current smokers than at those of non-smokers. In addition, those species were found in higher quantities at implants of subjects with periodontitis. The prevalence of Prevotella intermedia, Treponema denticola, C. rectus, and moreover of Staphylococcus warneri might be associated with peri-implant inflammation. Selected staphylococcal species (not Staphylococcus aureus), aerobic gram-negative rods, and yeasts were frequently detected, but with the exception of S. warneri, they did not show any association with periodontal or peri-implant diseases.
Smoking and periodontal disease are risk factors for colonization of periodontopathic bacteria at implants. Those bacterial species may play a potential role in peri-implant inflammation. The role of S. warneri needs further validation.
确定采用喷砂和酸蚀表面种植体植入10年后种植体及相邻牙齿处的微生物群。
使用基于核酸的方法，对从504颗种植体和493颗相邻牙齿的最深部位采集的菌斑样本进行分析，检测与牙周炎相关的某些细菌种类、葡萄球菌、需氧革兰氏阴性杆菌和酵母菌。
在6.2%-78.4%的种植体中可检测到已知与牙周炎相关的菌种。与牙齿相比，在种植体处检测到的牙龈卟啉单胞菌、微小单胞菌、具核梭杆菌/坏死梭杆菌和直肠弯曲菌数量显著更高。当前吸烟者种植体处可检测到的牙周致病菌种数量高于非吸烟者。此外，在患有牙周炎的受试者的种植体处发现这些菌种的数量更多。中间普氏菌、齿垢密螺旋体、直肠弯曲菌以及沃氏葡萄球菌的流行可能与种植体周围炎症有关。经常检测到选定的葡萄球菌种类（不包括金黄色葡萄球菌）、需氧革兰氏阴性杆菌和酵母菌，但除沃氏葡萄球菌外，它们与牙周或种植体周围疾病无任何关联。
吸烟和牙周疾病是种植体处牙周病原菌定植的危险因素。这些细菌种类可能在种植体周围炎症中发挥潜在作用。沃氏葡萄球菌的作用需要进一步验证。
