Abulnaja Khalid O, El Rabey Haddad A
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia ; Genetic Engineering and Biotechnology Institute, Sadat City University, P.O. Box 79, Sadat City, Minufiya, Egypt.
Evid Based Complement Alternat Med. 2015;2015:740716. doi: 10.1155/2015/740716. Epub 2015 Mar 17.
The current study focused on testing the hypolipidemic activity of two doses of barley bran on hypercholesterolemic male rats. Twenty-four male albino rats weighing 180-200 gm were divided into four groups. The first group (G1) was the negative control, the second group (G2) was the positive control group fed 2% cholesterol in the diet, and rats of the third and the fourth groups were fed 2% cholesterol and were cosupplemented with 5% and 10% barley bran, respectively, for 8 weeks. The hypercholesterolemic rats of (G2) showed an increase in lipid profile, liver enzymes, lactate dehydrogenase, creatine kinase-MB, and lipid peroxide and a decrease in antioxidant enzymes, whereas kidney function, fasting blood sugar, glycated hemoglobin total protein, and total bilirubin were not significantly affected compared with the negative control group in G1. Moreover, histology of heart, liver, and kidney of G2 rats showed histopathological changes compared with the negative control. Administration of the two doses of barley bran in G3 and G4 to the hypercholesterolemic rats ameliorated the level of lipids, liver enzymes, lactate dehydrogenase, and creatine kinase-MB. In addition, the histology of heart, liver, and kidney tissues nearly restored the normal state as in G1.
当前的研究聚焦于测试两剂大麦麸对高胆固醇血症雄性大鼠的降血脂活性。将24只体重为180 - 200克的雄性白化病大鼠分为四组。第一组（G1）为阴性对照组，第二组（G2）为在饮食中饲喂2%胆固醇的阳性对照组，第三组和第四组大鼠饲喂2%胆固醇，并分别同时补充5%和10%的大麦麸，持续8周。（G2）组的高胆固醇血症大鼠的血脂水平、肝酶、乳酸脱氢酶、肌酸激酶-MB和脂质过氧化物增加，而抗氧化酶减少，然而与G1组的阴性对照组相比，肾功能、空腹血糖、糖化血红蛋白、总蛋白和总胆红素未受到显著影响。此外，与阴性对照组相比，G2组大鼠的心脏、肝脏和肾脏组织学显示出组织病理学变化。给G3和G4组的高胆固醇血症大鼠施用两剂大麦麸改善了血脂、肝酶、乳酸脱氢酶和肌酸激酶-MB的水平。此外，心脏、肝脏和肾脏组织学几乎恢复到了G1组的正常状态。
