在无造影剂的情况下对活体小鼠视网膜中的半透明细胞体进行成像。
Imaging translucent cell bodies in the living mouse retina without contrast agents.
作者信息
Guevara-Torres A, Williams D R, Schallek J B
机构信息
Center for Visual Science, University of Rochester, Rochester, NY, 14627, USA ; The Institute of Optics, University of Rochester, Rochester, NY, 14620, USA.
Center for Visual Science, University of Rochester, Rochester, NY, 14627, USA ; The Institute of Optics, University of Rochester, Rochester, NY, 14620, USA ; Flaum Eye Institute, University of Rochester, Rochester, NY, 14642, USA.
出版信息
Biomed Opt Express. 2015 May 18;6(6):2106-19. doi: 10.1364/BOE.6.002106. eCollection 2015 Jun 1.
Abstract
The transparency of most retinal cell classes typically precludes imaging them in the living eye; unless invasive methods are used that deploy extrinsic contrast agents. Using an adaptive optics scanning light ophthalmoscope (AOSLO) and capitalizing on the large numerical aperture of the mouse eye, we enhanced the contrast from otherwise transparent cells by subtracting the left from the right half of the light distribution in the detector plane. With this approach, it is possible to image the distal processes of photoreceptors, their more proximal cell bodies and the mosaic of horizontal cells in the living mouse retina.
摘要
大多数视网膜细胞类型的透明度通常使其无法在活体眼中成像；除非使用部署外源性造影剂的侵入性方法。利用自适应光学扫描激光检眼镜（AOSLO）并借助小鼠眼睛的大数值孔径，我们通过从探测器平面光分布的右半部分减去左半部分来增强原本透明细胞的对比度。通过这种方法，可以对活体小鼠视网膜中光感受器的远端突起、更近端的细胞体以及水平细胞的镶嵌结构进行成像。
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