Zheng Bo, Vazin Tandis, Goodwill Patrick W, Conway Anthony, Verma Aradhana, Saritas Emine Ulku, Schaffer David, Conolly Steven M
Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.
Department of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.
Sci Rep. 2015 Sep 11;5:14055. doi: 10.1038/srep14055.
We demonstrate that Magnetic Particle Imaging (MPI) enables monitoring of cellular grafts with high contrast, sensitivity, and quantitativeness. MPI directly detects the intense magnetization of iron-oxide tracers using low-frequency magnetic fields. MPI is safe, noninvasive and offers superb sensitivity, with great promise for clinical translation and quantitative single-cell tracking. Here we report the first MPI cell tracking study, showing 200-cell detection in vitro and in vivo monitoring of human neural graft clearance over 87 days in rat brain.
我们证明，磁粒子成像（MPI）能够以高对比度、高灵敏度和定量性监测细胞移植。MPI使用低频磁场直接检测氧化铁示踪剂的强磁化。MPI安全、无创，具有极高的灵敏度，在临床转化和定量单细胞追踪方面极具前景。在此，我们报告了首个MPI细胞追踪研究，展示了在体外检测200个细胞以及在大鼠脑内对人类神经移植清除情况进行长达87天的体内监测。
