Department of Emergency Medicine, School of Medicine, Chosun University, Gwangju, Korea.
PLoS One. 2022 Jun 23;17(6):e0270281. doi: 10.1371/journal.pone.0270281. eCollection 2022.
In this study, a hydrogel comprising poly (vinyl alcohol)/pectin (PVA/PET) was prepared by the addition of Melia azedarach extract for epithelial restoration. M. azedarach extract (MAE) contains volatile organic plant-derived compounds with antimicrobial properties. MAE has a variety of physiological properties, including antimicrobial, insecticidal, and anti-inflammatory activity. This study aimed to investigate whether MAE-loaded PVA/PET hydrogels have protective effects against burn wound healing.
To mix M. azedarach with the gel, nanoparticles containing M. azedarach were prepared using chitosan/maltodextrin as the wall material. A PVA/PET hydrogel containing M. azedarach was developed and its applicability as a wound dressing was evaluated. In the in vitro scratch assay, MAE treatment showed a scratch recovery-promoting effect comparable to that of the positive control TGF-β1. The MAE-PVA/PET hydrogel was found to be non-toxic, and the antibacterial activity of the hydrogel was excellent against both gram-positive and gram-negative bacteria. Furthermore, as the formulated hydrogel demonstrated strong antimicrobial activity, its wound-healing efficacy was investigated in vivo using a rat model.
MAE was found to be effective against burn wounds and to have antimicrobial activity in vitro and in vivo.
本研究通过添加楝树提取物制备了一种包含聚乙烯醇/果胶（PVA/PET）的水凝胶，用于上皮修复。楝树提取物（MAE）含有具有抗菌特性的挥发性有机植物衍生化合物。MAE 具有多种生理特性，包括抗菌、杀虫和抗炎活性。本研究旨在探讨负载 MAE 的 PVA/PET 水凝胶是否对烧伤创面愈合具有保护作用。
为了将楝树与凝胶混合，使用壳聚糖/麦芽糊精作为壁材制备含有楝树的纳米颗粒。开发了一种含有 MAE 的 PVA/PET 水凝胶，并评估了其作为伤口敷料的适用性。在体外划痕实验中，MAE 处理表现出与阳性对照 TGF-β1 相当的划痕恢复促进作用。MAE-PVA/PET 水凝胶被发现是无毒的，并且对革兰氏阳性和革兰氏阴性菌均具有优异的抗菌活性。此外，由于所配制的水凝胶表现出很强的抗菌活性，因此使用大鼠模型在体内研究了其对烧伤创面的愈合效果。
MAE 对烧伤创面有效，并且在体外和体内均具有抗菌活性。
