末端螺旋展开作为稳定因子调节跨膜螺旋动力学。
Juxta-terminal Helix Unwinding as a Stabilizing Factor to Modulate the Dynamics of Transmembrane Helices.
机构信息
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, 72701, USA.
出版信息
Chembiochem. 2016 Mar 15;17(6):462-5. doi: 10.1002/cbic.201500656. Epub 2016 Feb 10.
Abstract
Transmembrane helices of integral membrane proteins often are flanked by interfacial aromatic residues that can serve as anchors to aid the stabilization of a tilted transmembrane orientation. Yet, physical factors that govern the orientation or dynamic averaging of individual transmembrane helices are not well understood and have not been adequately explained. Using solid-state (2) H NMR spectroscopy to examine lipid bilayer-incorporated model peptides of the GWALP23 (acetyl-GGALW(LA)6 LWLAGA-amide) family, we observed substantial unwinding at the terminals of several tilted helices spanning the membranes of DLPC, DMPC, or DOPC lipid bilayers. The fraying of helix ends might be vital for defining the dynamics and orientations of transmembrane helices in lipid bilayer membranes.
摘要
整合膜蛋白的跨膜螺旋通常被界面芳香族残基包围，这些残基可以作为锚定物来帮助稳定倾斜的跨膜取向。然而，控制单个跨膜螺旋取向或动态平均的物理因素尚未得到很好的理解，也没有得到充分的解释。我们使用固态 (2) H NMR 光谱法研究 GWALP23（乙酰-GGALW(LA)6 LWLAGA-酰胺）家族的脂质双层嵌入模型肽，观察到在跨越 DLPC、DMPC 或 DOPC 脂质双层膜的几个倾斜螺旋的末端存在大量解旋。螺旋末端的磨损对于定义脂质双层膜中跨膜螺旋的动力学和取向可能至关重要。
相似文献
1
Juxta-terminal Helix Unwinding as a Stabilizing Factor to Modulate the Dynamics of Transmembrane Helices.末端螺旋展开作为稳定因子调节跨膜螺旋动力学。
Chembiochem. 2016 Mar 15;17(6):462-5. doi: 10.1002/cbic.201500656. Epub 2016 Feb 10.
2
Comparisons of interfacial Phe, Tyr, and Trp residues as determinants of orientation and dynamics for GWALP transmembrane peptides.比较界面上的 Phe、Tyr 和 Trp 残基作为 GWALP 跨膜肽的取向和动力学决定因素。
Biochemistry. 2014 Jun 10;53(22):3637-45. doi: 10.1021/bi500439x. Epub 2014 May 29.
3
Proline kink angle distributions for GWALP23 in lipid bilayers of different thicknesses.GWALP23 在不同厚度脂质双层中的脯氨酸扭角分布。
Biochemistry. 2012 May 1;51(17):3554-64. doi: 10.1021/bi300281k. Epub 2012 Apr 18.
4
Influence of interfacial tryptophan residues on an arginine-flanked transmembrane helix.界面色氨酸残基对精氨酸侧翼跨膜螺旋的影响。
Biochim Biophys Acta Biomembr. 2020 Feb 1;1862(2):183134. doi: 10.1016/j.bbamem.2019.183134. Epub 2019 Nov 16.
5
Transmembrane Helix Integrity versus Fraying To Expose Hydrogen Bonds at a Membrane-Water Interface.跨膜螺旋的完整性与在膜-水界面处暴露氢键的磨损。
Biochemistry. 2019 Feb 12;58(6):633-645. doi: 10.1021/acs.biochem.8b01119. Epub 2019 Jan 3.
6
Influence of Lipid Saturation, Hydrophobic Length and Cholesterol on Double-Arginine-Containing Helical Peptides in Bilayer Membranes.双层膜中含二精氨酸螺旋肽的脂饱和度、疏水性长度和胆固醇的影响。
Chembiochem. 2019 Nov 4;20(21):2784-2792. doi: 10.1002/cbic.201900282. Epub 2019 Sep 18.
7
Control of Transmembrane Helix Dynamics by Interfacial Tryptophan Residues.通过界面色氨酸残基控制跨膜螺旋动力学。
Biophys J. 2018 Jun 5;114(11):2617-2629. doi: 10.1016/j.bpj.2018.04.016.
8
Tyrosine replacing tryptophan as an anchor in GWALP peptides.色氨酸被酪氨酸取代作为 GWALP 肽的锚点。
Biochemistry. 2012 Mar 13;51(10):2044-53. doi: 10.1021/bi201732e. Epub 2012 Mar 5.
9
Response of GWALP transmembrane peptides to changes in the tryptophan anchor positions.GWALP 跨膜肽对色氨酸锚定位点变化的响应。
Biochemistry. 2011 Sep 6;50(35):7522-35. doi: 10.1021/bi2006459. Epub 2011 Aug 12.
10
Influence of glutamic acid residues and pH on the properties of transmembrane helices.谷氨酸残基和 pH 值对跨膜螺旋性质的影响。
Biochim Biophys Acta Biomembr. 2017 Mar;1859(3):484-492. doi: 10.1016/j.bbamem.2017.01.006. Epub 2017 Jan 7.
引用本文的文献
1
Illuminating Disorder Induced by Glu in a Stable Arg-Anchored Transmembrane Helix.由谷氨酸在稳定的精氨酸锚定跨膜螺旋中诱导的光照紊乱
ACS Omega. 2021 Jul 26;6(31):20611-20618. doi: 10.1021/acsomega.1c02800. eCollection 2021 Aug 10.
2
Comparing Interfacial Trp, Interfacial His and pH Dependence for the Anchoring of Tilted Transmembrane Helical Peptides.比较倾斜跨膜螺旋肽的界面色氨酸、界面组氨酸和 pH 依赖性的锚固。
Biomolecules. 2020 Feb 11;10(2):273. doi: 10.3390/biom10020273.
3
Influence of interfacial tryptophan residues on an arginine-flanked transmembrane helix.界面色氨酸残基对精氨酸侧翼跨膜螺旋的影响。
Biochim Biophys Acta Biomembr. 2020 Feb 1;1862(2):183134. doi: 10.1016/j.bbamem.2019.183134. Epub 2019 Nov 16.
4
Breaking the Backbone: Central Arginine Residues Induce Membrane Exit and Helix Distortions within a Dynamic Membrane Peptide.打破脊梁：中央精氨酸残基诱导动态膜肽的膜出口和螺旋扭曲。
J Phys Chem B. 2019 Sep 26;123(38):8034-8047. doi: 10.1021/acs.jpcb.9b06034. Epub 2019 Sep 17.
5
Transmembrane Helix Integrity versus Fraying To Expose Hydrogen Bonds at a Membrane-Water Interface.跨膜螺旋的完整性与在膜-水界面处暴露氢键的磨损。
Biochemistry. 2019 Feb 12;58(6):633-645. doi: 10.1021/acs.biochem.8b01119. Epub 2019 Jan 3.
6
Wavelength-Selective Fluorescence of a Model Transmembrane Peptide: Constrained Dynamics of Interfacial Tryptophan Anchors.模型跨膜肽的波长选择荧光：界面色氨酸锚定的约束动力学。
J Fluoresc. 2018 Nov;28(6):1317-1323. doi: 10.1007/s10895-018-2293-5. Epub 2018 Sep 17.
7
Control of Transmembrane Helix Dynamics by Interfacial Tryptophan Residues.通过界面色氨酸残基控制跨膜螺旋动力学。
Biophys J. 2018 Jun 5;114(11):2617-2629. doi: 10.1016/j.bpj.2018.04.016.
8
Membrane Bending Moduli of Coexisting Liquid Phases Containing Transmembrane Peptide.含有跨膜肽的共存液相的膜弯曲模量。
Biophys J. 2018 May 8;114(9):2152-2164. doi: 10.1016/j.bpj.2018.03.026.
9
Influence of glutamic acid residues and pH on the properties of transmembrane helices.谷氨酸残基和 pH 值对跨膜螺旋性质的影响。
Biochim Biophys Acta Biomembr. 2017 Mar;1859(3):484-492. doi: 10.1016/j.bbamem.2017.01.006. Epub 2017 Jan 7.
10
Ionization Properties of Histidine Residues in the Lipid Bilayer Membrane Environment.脂质双分子层膜环境中组氨酸残基的电离特性
J Biol Chem. 2016 Sep 2;291(36):19146-56. doi: 10.1074/jbc.M116.738583. Epub 2016 Jul 20.
本文引用的文献
1
The dynamics of the G protein-coupled neuropeptide Y2 receptor in monounsaturated membranes investigated by solid-state NMR spectroscopy.通过固态核磁共振光谱研究单不饱和膜中G蛋白偶联神经肽Y2受体的动力学。
J Biomol NMR. 2015 Apr;61(3-4):347-59. doi: 10.1007/s10858-014-9892-5. Epub 2015 Jan 6.
2
Comparisons of interfacial Phe, Tyr, and Trp residues as determinants of orientation and dynamics for GWALP transmembrane peptides.比较界面上的 Phe、Tyr 和 Trp 残基作为 GWALP 跨膜肽的取向和动力学决定因素。
Biochemistry. 2014 Jun 10;53(22):3637-45. doi: 10.1021/bi500439x. Epub 2014 May 29.
3
Molecular signatures of G-protein-coupled receptors.G 蛋白偶联受体的分子特征。
Nature. 2013 Feb 14;494(7436):185-94. doi: 10.1038/nature11896.
4
Knowledge-based potential for positioning membrane-associated structures and assessing residue-specific energetic contributions.基于知识的定位膜相关结构和评估残基特异性能量贡献的潜力。
Structure. 2012 May 9;20(5):924-35. doi: 10.1016/j.str.2012.03.016.
5
Tyrosine replacing tryptophan as an anchor in GWALP peptides.色氨酸被酪氨酸取代作为 GWALP 肽的锚点。
Biochemistry. 2012 Mar 13;51(10):2044-53. doi: 10.1021/bi201732e. Epub 2012 Mar 5.
6
Hydrophobic mismatch of mobile transmembrane helices: Merging theory and experiments.可移动跨膜螺旋的疏水不匹配：理论与实验的融合
Biochim Biophys Acta. 2012 May;1818(5):1242-9. doi: 10.1016/j.bbamem.2012.01.023. Epub 2012 Feb 2.
7
On the combined analysis of ²H and ¹⁵N/¹H solid-state NMR data for determination of transmembrane peptide orientation and dynamics.基于 ²H 和 ¹⁵N/¹H 固体核磁共振数据分析的跨膜肽取向和动力学的联合分析。
Biophys J. 2011 Dec 21;101(12):2939-47. doi: 10.1016/j.bpj.2011.11.008. Epub 2011 Dec 20.
8
In silico partitioning and transmembrane insertion of hydrophobic peptides under equilibrium conditions.在平衡条件下疏水肽的计算分区和跨膜插入。
J Am Chem Soc. 2011 Oct 5;133(39):15487-95. doi: 10.1021/ja204042f. Epub 2011 Sep 14.
9
Side-chain hydrophobicity scale derived from transmembrane protein folding into lipid bilayers.侧链疏水性尺度来源于跨膜蛋白折叠到脂质双层中。
Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10174-7. doi: 10.1073/pnas.1103979108. Epub 2011 May 23.
10
Membrane interface composition drives the structure and the tilt of the single transmembrane helix protein PMP1: MD studies.膜界面组成决定单跨膜螺旋蛋白 PMP1 的结构和倾斜：MD 研究。
Biophys J. 2011 Apr 6;100(7):1660-7. doi: 10.1016/j.bpj.2011.02.002.
Zotero 插件