Surana Sunaina, Shenoy Avinash R, Krishnan Yamuna
Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, 60637 Illinois, USA.
Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK.
Nat Nanotechnol. 2015 Sep;10(9):741-7. doi: 10.1038/nnano.2015.180.
DNA is proving to be a powerful scaffold to construct molecularly precise designer DNA devices. Recent trends reveal their ever-increasing deployment within living systems as delivery devices that not only probe but also program and re-program a cell, or even whole organisms. Given that DNA is highly immunogenic, we outline the molecular, cellular and organismal response pathways that designer nucleic acid nanodevices are likely to elicit in living systems. We address safety issues applicable when such designer DNA nanodevices interact with the immune system. In light of this, we discuss possible molecular programming strategies that could be integrated with such designer nucleic acid scaffolds to either evade or stimulate the host response with a view to optimizing and widening their applications in higher organisms.
事实证明，DNA是构建分子精确的定制DNA装置的强大支架。最近的趋势表明，它们在生命系统中的应用越来越广泛，作为递送装置，不仅可以探测细胞，还可以对细胞甚至整个生物体进行编程和重新编程。鉴于DNA具有高度免疫原性，我们概述了定制核酸纳米装置在生命系统中可能引发的分子、细胞和生物体反应途径。我们讨论了此类定制DNA纳米装置与免疫系统相互作用时适用的安全问题。有鉴于此，我们讨论了可能与此类定制核酸支架整合的分子编程策略，以规避或刺激宿主反应，从而优化并扩大它们在高等生物中的应用。
