Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Sci Rep. 2016 Jun 2;6:27226. doi: 10.1038/srep27226.
Photocurable, biocompatible liquid resins are highly desired for 3D stereolithography based bioprinting. Here we solidified a novel renewable soybean oil epoxidized acrylate, using a 3D laser printing technique, into smart and highly biocompatible scaffolds capable of supporting growth of multipotent human bone marrow mesenchymal stem cells (hMSCs). Porous scaffolds were readily fabricated by simply adjusting the printer infill density; superficial structures of the polymerized soybean oil epoxidized acrylate were significantly affected by laser frequency and printing speed. Shape memory tests confirmed that the scaffold fixed a temporary shape at -18 °C and fully recovered its original shape at human body temperature (37 °C), which indicated the great potential for 4D printing applications. Cytotoxicity analysis proved that the printed scaffolds had significant higher hMSC adhesion and proliferation than traditional polyethylene glycol diacrylate (PEGDA), and had no statistical difference from poly lactic acid (PLA) and polycaprolactone (PCL). This research is believed to significantly advance the development of biomedical scaffolds with renewable plant oils and advanced 3D fabrication techniques.
用于基于立体光固化技术的生物打印的光固化、生物相容的液态树脂备受期待。在这里，我们使用 3D 激光打印技术将新型可再生的大豆油环氧化丙烯酸酯固化成智能且高度生物相容的支架，能够支持多能人类骨髓间充质干细胞（hMSC）的生长。通过简单地调整打印机填充密度，即可轻松制备多孔支架；聚合的大豆油环氧化丙烯酸酯的表面结构受到激光频率和打印速度的显著影响。形状记忆测试证实，支架在-18°C 时固定临时形状，并在人体温度（37°C）下完全恢复原始形状，这表明其在 4D 打印应用方面具有巨大潜力。细胞毒性分析表明，与传统的聚乙二醇二丙烯酸酯（PEGDA）相比，打印支架显著提高了 hMSC 的黏附率和增殖率，与聚乳酸（PLA）和聚己内酯（PCL）没有统计学差异。这项研究有望显著推进使用可再生植物油和先进的 3D 制造技术开发生物医学支架。
