Kang Hae Ji, Gu Se Hun, Cook Joseph A, Yanagihara Richard
Pacific Center for Emerging Infectious Diseases Research, Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI USA.
Museum of Southwestern Biology, Department of Biology, University of New Mexico, Albuquerque, NM USA.
Trop Med Health. 2016 Jun 20;44:16. doi: 10.1186/s41182-016-0017-6. eCollection 2016.
Novel hantaviruses, recently detected in moles (order Eulipotyphla, family Talpidae) from Europe, Asia, and North America would predict a broader host range and wider ecological diversity. Employing RT-PCR, archival frozen tissues from the Chinese shrew mole (Uropsilus soricipes), broad-footed mole (Scapanus latimanus), coast mole (Scapanus orarius), Townsend's mole (Scapanus townsendii), and long-tailed mole (Scaptonyx fusicaudus) were analyzed for hantavirus RNA. Following multiple attempts, a previously unrecognized hantavirus, designated Dahonggou Creek virus (DHCV), was detected in a long-tailed mole, captured in Shimian County, Sichuan Province, People's Republic of China, in August 1989. Analyses of a 1058-nucleotide region of the RNA-dependent RNA polymerase-encoding L segment indicated that DHCV was genetically distinct from other rodent-, shrew-, mole-, and bat-borne hantaviruses. Phylogenetic trees, using maximum likelihood and Bayesian methods, showed that DHCV represented a divergent lineage comprising crocidurine and myosoricine shrew-borne hantaviruses. Although efforts to obtain the S- and M-genomic segments failed, the L-segment sequence analysis, reported here, expands the genetic database of non-rodent-borne hantaviruses. Also, by further mining natural history collections of archival specimens, the genetic diversity of hantaviruses will elucidate their evolutionary origins.
最近在欧洲、亚洲和北美的鼹鼠（真盲缺目，鼹科）中检测到的新型汉坦病毒预示着更广泛的宿主范围和更丰富的生态多样性。利用逆转录聚合酶链反应（RT-PCR），对中国鼩鼹（Uropsilus soricipes）、阔脚鼹（Scapanus latimanus）、海岸鼹（Scapanus orarius）、汤森氏鼹（Scapanus townsendii）和长尾鼹（Scaptonyx fusicaudus）的存档冷冻组织进行了汉坦病毒RNA分析。经过多次尝试，在1989年8月于中华人民共和国四川省石棉县捕获的一只长尾鼹中检测到一种先前未被识别的汉坦病毒，命名为大洪沟溪病毒（DHCV）。对RNA依赖的RNA聚合酶编码L片段的1058个核苷酸区域的分析表明，DHCV在基因上与其他啮齿动物、鼩鼱、鼹鼠和蝙蝠传播的汉坦病毒不同。使用最大似然法和贝叶斯方法构建的系统发育树表明，DHCV代表了一个不同的谱系，包括由麝鼩和白齿麝鼩传播的汉坦病毒。尽管获取S和M基因组片段的努力失败了，但本文报道的L片段序列分析扩展了非啮齿动物传播的汉坦病毒的基因数据库。此外，通过进一步挖掘存档标本的自然历史收藏，汉坦病毒的遗传多样性将阐明它们的进化起源。
