Zheng Xiaofen, Yao Juan, Li Bing
Department of Corneal Diseases, Shanxi Eye Hospital, Fu Dong Street No. 100, Xinghualing District, Taiyuan City, Shanxi Province, China.
J Ophthalmol. 2016;2016:5072781. doi: 10.1155/2016/5072781. Epub 2016 Jul 18.
Background. The pathogenesis of allergic conjunctivitis has not been clearly established. Moreover, previous studies fail to consider human models of allergic conjunctivitis. This study investigated the expression of thymic stromal lymphopoiet in TSLP and its downstream molecules in conjunctival scrappings and tear. Methods. This cross-sectional study compares patients with vernal keratoconjunctivitis (VKC), seasonal allergic conjunctivitis (SAC), and perennial allergic conjunctivitis (PAC) with normal controls. There are 80 people recorded in Shanxi Eye Hospital. Increasingly, 20 are with VKC, 20 are with SAC, 20 are with PAC, and the remaining 20 are normal controls. Conjunctiva were harvested for total RNA extraction and gene expression by real-time polymerase chain reaction. Epithelial cells were collected to make pathological sections for immunohistochemical staining. Human tears were evaluated by Luminex microbead assay. A P value less than 0.05 from Dunnett's post hoc test in SPSS means a statistical significant distinction. Results. Positive expression in conjunctival cells of patients with allergic conjunctivitis. The expression of TSLP and IL-4, IL-5, and IL-13 mRNA shows a statistically significant difference (P < 0.05). TSLP and IL-4, IL-5, and IL-13 concentrations show a statistically significant difference (P < 0.01). Conclusions. This study suggests that TSLP and downstream molecules are expressed in patients with various types of allergic conjunctivitis.
背景。过敏性结膜炎的发病机制尚未明确确立。此外，以往研究未考虑过敏性结膜炎的人体模型。本研究调查了胸腺基质淋巴细胞生成素（TSLP）及其下游分子在结膜刮片和泪液中的表达。方法。这项横断面研究将春季角结膜炎（VKC）、季节性过敏性结膜炎（SAC）和常年性过敏性结膜炎（PAC）患者与正常对照进行比较。山西眼科医院记录了80人。其中，20人患有VKC，20人患有SAC，20人患有PAC，其余20人为正常对照。采集结膜用于提取总RNA并通过实时聚合酶链反应进行基因表达分析。收集上皮细胞制作病理切片进行免疫组织化学染色。通过Luminex微珠分析评估人泪液。SPSS中Dunnett事后检验的P值小于0.05表示有统计学显著差异。结果。过敏性结膜炎患者结膜细胞呈阳性表达。TSLP以及IL-4、IL-5和IL-13 mRNA的表达有统计学显著差异（P < 0.05）。TSLP以及IL-4、IL-5和IL-13的浓度有统计学显著差异（P < 0.01）。结论。本研究表明，TSLP及其下游分子在各类过敏性结膜炎患者中均有表达。
