Department of Biological Sciences, Applied Biotechnology and Genetic Engineering Lab, International Islamic University, Islamabad, Pakistan.
Department of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.
PLoS One. 2020 Jul 9;15(7):e0230464. doi: 10.1371/journal.pone.0230464. eCollection 2020.
The current study focuses on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the effect of zinc oxide nanoparticles on plant regeneration ability in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive spectroscopy X-ray (EDS) depicted major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO-NPs demonstrated the incredibly promising results against callogenesis. Biosynthesized ZnO-NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/L both explants demonstrated 70% and 76% regeneration frequency. The results conclude that ZnO-NPs enhance the plant growth and development and tailored the nutritive properties at nano-scale. Furthermore, eco-friendly approach of ZnO-NPs synthesis is strongly believed to improve in vitro regeneration frequencies in several other monocot plants.
本研究专注于使用生物合成的氧化锌纳米粒子来提高重要饲料草柳枝稷的组织培养效率。锌作为一种微量元素，以不同浓度增强了柳枝稷的细胞发生和再生效率。在这里，我们通过香茅草叶片提取物合成了氧化锌纳米粒子，以评估氧化锌纳米粒子对柳枝稷植物再生能力的影响。X 射线衍射（XRD）和衰减全反射-傅里叶变换红外（ATR-FTIR）验证了绿色合成氧化锌纳米粒子的相纯度，而电子显微镜（SEM）则说明了颗粒的平均尺寸为 50±4nm，呈六方柱状。能谱 X 射线（EDS）描绘了 Zn（92.68%）的主要峰，而次要峰则表示氧（7.32%）。ZnO-NPs 对细胞发生表现出非常有前景的结果。在最佳浓度下合成的生物 ZnO-NPs 对植物再生能力表现出非常有希望的效果。两种外植体，种子和节点都表现出剂量依赖性反应，超过 40mg/L 时，结果记录为阴性，而在 30mg/L 时，两种外植体的再生频率都达到了 70%和 76%。结果表明，ZnO-NPs 增强了植物的生长和发育，并在纳米尺度上调整了营养特性。此外，强烈相信 ZnO-NPs 的环保合成方法将提高其他几种单子叶植物的体外再生频率。
