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白杨素-PLGA-PEG纳米颗粒对胃癌细胞系中miRNAs增殖和基因表达的影响

Effects of Chrysin-PLGA-PEG Nanoparticles on Proliferation and Gene Expression of miRNAs in Gastric Cancer Cell Line.

作者信息

Mohammadian Farideh, Abhari Alireza, Dariushnejad Hassan, Nikanfar Alireza, Pilehvar-Soltanahmadi Younes, Zarghami Nosratollah

机构信息

Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IR Iran.

Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.

出版信息

Iran J Cancer Prev. 2016 Aug 16;9(4):e4190. doi: 10.17795/ijcp-4190. eCollection 2016 Aug.

DOI:10.17795/ijcp-4190
PMID:27761206
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5056017/
Abstract

BACKGROUND

Recently, Chrysin, as a flavone, has revealed cancer chemo-preventive activity. The present experiment utilized the PLGA-PEG-chrysin complex, and free chrysin, to evaluation of the expression of miR-22, miR-34a and miR-126 in human gastric cell line.

OBJECTIVES

The purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on AGS human gastric cell line.

METHODS

Properties of the chrysin encapsulated in PLGA-PEG nanoparticles were investigated by SEM, H NMR, and FTIR. The assessment of cytotoxicity on the growth of the human gastric cell line was carried out through MTT assay. After treating the cells with a prearranged amount of pure and encapsulated chrysin, RNA was extracted and the expressions of miR-22, miR-34a and miR-126 were measured by using real-time PCR.

RESULTS

With regard to the amount of the chrysin loaded in PLGA-PEG nanoparticles, IC50 value was significantly decreased in nanocapsulatedchrysin, in comparison with free chrysin. This finding has been proved through the further increase of miR-22, miR-34a and miR-126 gene expression of nanocapsulatedchrysin, in comparison with free chrysin.

CONCLUSIONS

In this study, we revealed that the PLGA-PEG-chrysin is more effective than free chrysin in inhibiting the growth of human gastric cell line.

摘要

背景

最近，白杨素作为一种黄酮类化合物，已显示出癌症化学预防活性。本实验利用聚乳酸-乙醇酸共聚物-聚乙二醇-白杨素复合物和游离白杨素，评估人胃癌细胞系中miR-22、miR-34a和miR-126的表达。

目的

本研究旨在探讨纳米包裹的白杨素是否能增强游离白杨素对AGS人胃癌细胞系的抗癌作用。

方法

通过扫描电子显微镜（SEM）、核磁共振氢谱（1H NMR）和傅里叶变换红外光谱（FTIR）研究聚乳酸-乙醇酸共聚物-聚乙二醇纳米粒包裹的白杨素的性质。通过MTT法评估对人胃癌细胞系生长的细胞毒性。用预定剂量的纯白杨素和包裹的白杨素处理细胞后，提取RNA，并用实时聚合酶链反应（PCR）检测miR-22、miR-34a和miR-126的表达。

结果

就聚乳酸-乙醇酸共聚物-聚乙二醇纳米粒中负载的白杨素量而言，与游离白杨素相比，纳米包裹的白杨素的半数抑制浓度（IC50）值显著降低。与游离白杨素相比，纳米包裹的白杨素中miR-22、miR-34a和miR-126基因表达的进一步增加证明了这一发现。

结论

在本研究中，我们发现聚乳酸-乙醇酸共聚物-聚乙二醇-白杨素在抑制人胃癌细胞系生长方面比游离白杨素更有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/1bb7a18e65d1/ijcp-09-04-4190-i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/f265f7b57ec5/ijcp-09-04-4190-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/eaa5c2f16c65/ijcp-09-04-4190-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/bfedb1331c64/ijcp-09-04-4190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/b685fe3ada52/ijcp-09-04-4190-i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/1bb7a18e65d1/ijcp-09-04-4190-i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/f265f7b57ec5/ijcp-09-04-4190-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/eaa5c2f16c65/ijcp-09-04-4190-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/bfedb1331c64/ijcp-09-04-4190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/b685fe3ada52/ijcp-09-04-4190-i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0399/5056017/1bb7a18e65d1/ijcp-09-04-4190-i004.jpg

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