Jeun Minhong, Park Sungwook, Lee Hakho, Lee Kwan Hyi
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul.
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea.
Int J Nanomedicine. 2016 Oct 21;11:5497-5503. doi: 10.2147/IJN.S118156. eCollection 2016.
Magnetic-based biosensors are attractive for on-site detection of biomarkers due to the low magnetic susceptibility of biological samples. Here, we report a highly sensitive magnetic-based biosensing system that is composed of a miniaturized nuclear magnetic resonance (NMR) device and magnetically engineered nanoferrite particles (NFPs). The sensing performance, also identified as the transverse relaxation () rate, of the NMR device is directly related to the magnetic properties of the NFPs. Therefore, we developed magnetically engineered NFPs (MnMg-NFP) and used them as NMR agents to exhibit a significantly improved rate. The magnetization of the MnMg-NFPs was increased by controlling the Mn and Mg cation concentration and distribution during the synthesis process. This modification of the Mn and Mg cation directly contributed to improving the rate. The miniaturized NMR system, combined with the magnetically engineered MnMg-NFPs, successfully detected a small amount of infectious influenza A H1N1 nucleoprotein with high sensitivity and stability.
基于磁性的生物传感器因生物样品的低磁化率而对生物标志物的现场检测具有吸引力。在此，我们报告一种高度灵敏的基于磁性的生物传感系统，该系统由一个小型化核磁共振（NMR）装置和磁性工程化纳米铁氧体颗粒（NFPs）组成。NMR装置的传感性能，也被确定为横向弛豫（）速率，与NFPs的磁性直接相关。因此，我们开发了磁性工程化NFPs（MnMg-NFP）并将其用作NMR试剂以显著提高 速率。通过在合成过程中控制Mn和Mg阳离子的浓度和分布，MnMg-NFPs的磁化强度得以提高。Mn和Mg阳离子的这种修饰直接有助于提高 速率。结合磁性工程化MnMg-NFPs的小型化NMR系统成功地以高灵敏度和稳定性检测到少量传染性甲型H1N1流感核蛋白。
