Dornbusch K
Scand J Infect Dis Suppl. 1978(14):177-85.
With regard to determination of antibiotic levels in bone, variance in results may be obtained as a consequence of type of the bones involved, degree of penetration of the drug used and of assay method performed. Measurements of antibacterial activity in bone from patients undergoing orthopaedic surgery were performed after preoperative treatment with doxycycline, lincomycin, clindamycin or cefuroxime. In performing the assay by the agar diffusion method, after extraction of the bone specimens in buffer according to classical systems, the concentrations of doxycyline were in the range of 0.05--0.6 microgram/g, clindamycin 0.4--4.9 microgram/g, lincomycin 1.7--2.9 microgram/g and of cefuroxine about 0.4 microgram/g. After electrophoresis in agarose-gel and followed by microbiological assay higher levels of these drugs were estimated possibly as a consequence of a diminution in the binding capacity of bone.
关于骨骼中抗生素水平的测定，由于所涉及骨骼的类型、所用药物的渗透程度以及所采用的检测方法，可能会得到不同的结果。对接受骨科手术的患者，在术前使用强力霉素、林可霉素、克林霉素或头孢呋辛进行治疗后，对其骨骼中的抗菌活性进行了测量。采用琼脂扩散法进行检测时，按照传统方法在缓冲液中提取骨标本后，强力霉素的浓度范围为0.05 - 0.6微克/克，克林霉素为0.4 - 4.9微克/克，林可霉素为1.7 - 2.9微克/克，头孢呋辛约为0.4微克/克。在琼脂糖凝胶中进行电泳，随后进行微生物检测后，估计这些药物的水平较高，这可能是由于骨骼结合能力降低所致。
