Sander Lane C, Byrd Gary D
National Institute of Standards and Technology, Gaithersburg, MD 20899.
J Res Natl Inst Stand Technol. 1989 Sep-Oct;94(5):305-309. doi: 10.6028/jres.094.028.
A cotinine in freeze-dried urine reference material (RM 8444) was prepared at three concentrations: (1) a "blank" level typical of nonsmokers with no exposure to cigarette smoke, (2) a "low" level corresponding to nonsmokers with passive exposure to side-stream smoke, and (3) a "high" level typical of smokers. Low- and high-level materials were prepared gravimetrically from pooled urine by the addition of appropriate amounts of cotinine Perchlorate. Cotinine was determined by GC-MS using cotinine-) as an internal standard. No evidence for sample inhomogeneity was observed. This reference material will fulfill a need for a urine-based standard to assist in the validation of field methods used for assessing exposure to cigarette smoke.
冻干尿参考物质（RM 8444）中的可替宁制备了三种浓度：（1）无接触香烟烟雾的非吸烟者典型的“空白”水平；（2）对应于被动接触侧流烟雾的非吸烟者的“低”水平；（3）吸烟者典型的“高”水平。通过加入适量的高氯酸可替宁，采用重量法从混合尿中制备低水平和高水平物质。使用可替宁-d3作为内标，通过气相色谱-质谱法测定可替宁。未观察到样品不均匀性的证据。该参考物质将满足对基于尿液的标准的需求，以协助验证用于评估接触香烟烟雾的现场方法。
