González Ana M, Yuste-Lisbona Fernando J, Saburido Soledad, Bretones Sandra, De Ron Antonio M, Lozano Rafael, Santalla Marta
Grupo de Biología de Agrosistemas, Misión Biológica de Galicia-Consejo Superior de Investigaciones Cientificas Pontevedra, Spain.
Departamento de Biología y Geología (Genética), Centro de Investigación en Biotecnología Agroalimentaria, Universidad de Almería Almería, Spain.
Front Plant Sci. 2016 Dec 26;7:1940. doi: 10.3389/fpls.2016.01940. eCollection 2016.
Determinacy growth habit and accelerated flowering traits were selected during or after domestication in common bean. Both processes affect several presumed adaptive traits such as the rate of plant production. There is a close association between flowering initiation and vegetative growth; however, interactions among these two crucial developmental processes and their genetic bases remain unexplored. In this study, with the aim to establish the genetic relationships between these complex processes, a multi-environment quantitative trait locus (QTL) mapping approach was performed in two recombinant inbred line populations derived from inter-gene pool crosses between determinate and indeterminate genotypes. Additive and epistatic QTLs were found to regulate flowering time, vegetative growth, and rate of plant production. Moreover, the pleiotropic patterns of the identified QTLs evidenced that regions controlling time to flowering traits, directly or indirectly, are also involved in the regulation of plant production traits. Further QTL analysis highlighted one QTL, on the lower arm of the linkage group Pv01, harboring the gene, homologous to the () gene, which explained up to 32% of phenotypic variation for time to flowering, 66% for vegetative growth, and 19% for rate of plant production. This finding was consistent with previous results, which have also suggested ) as a candidate gene for determinacy locus. The information here reported can also be applied in breeding programs seeking to optimize key agronomic traits, such as time to flowering, plant height and an improved reproductive biomass, pods, and seed size, as well as yield.
菜豆在驯化过程中或驯化后选择了有限生长习性和加速开花性状。这两个过程都会影响一些假定的适应性性状，如植物生长速率。开花起始与营养生长之间存在密切联系；然而，这两个关键发育过程之间的相互作用及其遗传基础仍未得到探索。在本研究中，为了建立这些复杂过程之间的遗传关系，在两个重组自交系群体中采用了多环境数量性状位点（QTL）定位方法，这两个群体源自有限和无限基因型之间的基因池间杂交。发现加性和上位性QTL调控开花时间、营养生长和植物生长速率。此外，所鉴定QTL的多效性模式表明，直接或间接控制开花时间性状的区域也参与植物生产性状的调控。进一步的QTL分析突出了一个位于连锁群Pv01下臂的QTL，其包含与（）基因同源的基因，该基因解释了高达32%的开花时间表型变异、66%的营养生长表型变异和19%的植物生长速率表型变异。这一发现与之前的结果一致，之前的结果也表明）是有限生长位点的候选基因。这里报道的信息也可应用于旨在优化关键农艺性状的育种计划，如开花时间、株高以及改善生殖生物量、豆荚和种子大小以及产量。
