Zhang Liuhong, Niaz Shah Iram, Khan Dilfaraz, Wang Zhen, Zhu Yonghong, Zhou Haiyun, Lin Yongcheng, Li Jing, Liu Lan
School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510275, China.
Mar Drugs. 2017 Feb 10;15(2):35. doi: 10.3390/md15020035.
Two new sesquiterpenes, microsphaeropsisin B () and C (), and two new de--methyllasiodiplodins, (3, 7)-7-hydroxy-de--methyllasiodiplodin () and (3)-5-oxo-de--methyllasiodiplodin (), together with one new natural product () and twelve known compounds (, -), were isolated from the co-cultivation of mangrove endophytic fungus sp. 307 and aquatic pathogenic bacterium B2. Their structures, including absolute configurations, were elucidated by extensive analysis of spectroscopic data, electronic circular dichroism, Mo₂(AcO)₄-induced circular dichroism, and comparison with reported data. All of the isolated compounds were tested for their α-glucosidase inhibitory activity and cytotoxicity. New compounds and exhibited potent α-glucosidase inhibitory activity with IC values of 25.8 and 54.6 µM, respectively, which were more potent than the positive control (acarbose, IC = 703.8 µM). The good results of the tested bioactivity allowed us to explore α-glucosidase inhibitors in lasiodiplodins.
从红树林内生真菌sp. 307与水生致病细菌B2的共培养物中分离出两种新的倍半萜类化合物，即微球孢菌素B（）和C（），以及两种新的去甲基拉索地菌素，即(3, 7)-7-羟基去甲基拉索地菌素（）和(3)-5-氧代去甲基拉索地菌素（），还有一种新的天然产物（）和十二种已知化合物（， -）。通过对光谱数据、电子圆二色性、Mo₂(AcO)₄诱导的圆二色性进行广泛分析，并与已报道的数据进行比较，阐明了它们的结构，包括绝对构型。对所有分离出的化合物进行了α-葡萄糖苷酶抑制活性和细胞毒性测试。新化合物和表现出较强的α-葡萄糖苷酶抑制活性，IC值分别为25.8和54.6 μM，比阳性对照（阿卡波糖，IC = 703.8 μM）更强。所测试的生物活性的良好结果使我们能够探索拉索地菌素中的α-葡萄糖苷酶抑制剂。
