Boskabady Mohammad Hossein, Hashemzehi Milad, Khazdair Mohammad Reza, Askari Vahid Reza
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
pharmaceutical Research Center and Department of Physiology School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Pharm Res. 2016 Fall;15(4):867-874.
Thestimulatory effect of the extract of () on β-adrenoceptor of tracheal smooth muscle was examined.To examine β-adrenoceptor stimulatory effect, concentration response curve to isoprenaline was obtained in pre-contracted tracheal smooth muscle in the presence of three concentrations of aqueous-ethanolic extract, propranolol, and saline. Values of EC (the effective concentration of isoprenaline, causing 50% of maximum response) and dose ratio-1(CR-1) were measured. This effect was tested innon-incubated tracheal smooth muscle (group 1) and incubated tissues with chlorpheniramine (group 2). Concentration-response curves to isoprenaline in the presence of two higher concentrations of the extract in group 1 and all three concentrations in group 2 showed leftward shifts compared to isoprenaline curves produced in the presence of saline in both groups. EC obtained in the presence of propranolol was significantly higher than that of saline in both groups of experiments (p<0.05 for both cases). However, the EC obtained in the presence of two higher concentrations of the extract in group 1 and lower concentration in group 2 were non-significantly but those obtained of medium and high extract concentrations in the group 2 were significantly (p<0.05 for both cases)lower than those of saline. The values of (CR-1) obtained in the presence of all concentrations of the extract in groups1 and 2 were significantly lower than that of propranolol (p<0.05 to p<0.001).The results indicated a stimulatory effect of the extract on ß -adrenoceptors of tracheal smooth muscle.
研究了（）提取物对气管平滑肌β-肾上腺素能受体的刺激作用。为检测β-肾上腺素能受体刺激作用，在预先收缩的气管平滑肌中，于三种浓度的水乙醇提取物、普萘洛尔和生理盐水存在的情况下，获得了异丙肾上腺素的浓度-反应曲线。测量了EC（引起最大反应50%的异丙肾上腺素有效浓度）和剂量比-1（CR（CR-1的值。在未孵育的气管平滑肌（第1组）和用氯苯那敏孵育的组织（第2组）中测试了这种作用。与两组中生理盐水存在时产生的异丙肾上腺素曲线相比，第1组中两种较高浓度提取物存在时以及第2组中所有三种浓度提取物存在时，异丙肾上腺素的浓度-反应曲线均向左移动。在两组实验中，普萘洛尔存在时获得的EC均显著高于生理盐水（两种情况均p<0.05）。然而，第1组中两种较高浓度提取物存在时以及第2组中较低浓度提取物存在时获得的EC无显著差异，但第2组中提取物中浓度和高浓度时获得的EC显著低于生理盐水（两种情况均p<0.05）。第1组和第2组中所有浓度提取物存在时获得的（CR-1）值均显著低于普萘洛尔（p<0.05至p<0.001）。结果表明该提取物对气管平滑肌的β-肾上腺素能受体有刺激作用。
