Halbert C L, Galloway D A
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
J Virol. 1988 Mar;62(3):1071-5. doi: 10.1128/JVI.62.3.1071-1075.1988.
Sequencing of the E5 open reading frame (ORF) of human papillomavirus type 16 revealed an additional nucleotide, a thymidine residue, at position 3903 compared with the original sequence (Seedorf et al., Virology 145:181-185, 1985). The additional T had two effects; first, in reading frame 2, in which the original E5 ORF was predicted, the additional T changed the reading frame downstream of position 3903 to create an ORF, which we designated E5, that terminated at position 4018 and potentially encoded a 52-amino-acid polypeptide. Secondly, in reading frame 3, a new ORF was created (positions 3807 to 4097), which we propose is the authentic papillomavirus type 16 E5 ORF. It contained a methionine residue and encoded an additional 82 amino acids. Both ORFs have been cloned into bacterial expression vectors (pATH), and the fusion proteins have been used to generate polyclonal antibodies in rabbits.
人乳头瘤病毒16型E5开放阅读框（ORF）的测序结果显示，与原始序列（Seedorf等人，《病毒学》145:181 - 185，1985年）相比，在3903位有一个额外的核苷酸，即胸腺嘧啶残基。这个额外的T有两个影响：第一，在预测原始E5 ORF的阅读框2中，额外的T改变了3903位下游的阅读框，从而产生了一个ORF，我们将其命名为E5，该ORF在4018位终止，并可能编码一个52个氨基酸的多肽。第二，在阅读框3中，产生了一个新的ORF（3807至4097位），我们认为这是真正的人乳头瘤病毒16型E5 ORF。它含有一个甲硫氨酸残基，并编码另外82个氨基酸。这两个ORF都已被克隆到细菌表达载体（pATH）中，并且融合蛋白已被用于在兔子体内产生多克隆抗体。
