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伯氏疏螺旋体一种广泛存在于多种细菌中的60千道尔顿免疫显性抗原的免疫化学特性鉴定及该抗原基因的分离

Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria.

作者信息

Hansen K, Bangsborg J M, Fjordvang H, Pedersen N S, Hindersson P

机构信息

Department of Treponematoses, Statens Seruminstitut, Copenhagen, Denmark.

出版信息

Infect Immun. 1988 Aug;56(8):2047-53. doi: 10.1128/iai.56.8.2047-2053.1988.

DOI:10.1128/iai.56.8.2047-2053.1988
PMID:2840400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259521/
Abstract

By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi genomic library was constructed by using a plasmid cloning system. Escherichia coli recombinants were screened for expression of immunodominant B. burgdorferi antigens. One of the recombinant clones expressed the 60-kilodalton CA of B. burgdorferi. The DNA region encoding B. burgdorferi CA was localized on a 2.3-kilobase fragment of the plasmid pKH1. CA may have pathogenetic implications in Lyme borreliosis, since the CA of mycobacteria recently has been shown to play a role in the etiology of experimental autoimmune arthritis. The extensive cross-reactivity of this antigen may account for the low diagnostic specificity of the currently used serological tests in Lyme borreliosis.

摘要

通过交叉免疫电泳和蛋白质印迹法（免疫印迹法）显示，伯氏疏螺旋体表达60千道尔顿的共同抗原（CA），该抗原与多种远缘相关细菌中的等效抗原具有交叉反应性。伯氏疏螺旋体CA具有强烈的免疫原性。使用质粒克隆系统构建了伯氏疏螺旋体基因组文库。筛选大肠杆菌重组体以检测免疫显性伯氏疏螺旋体抗原的表达。其中一个重组克隆表达了伯氏疏螺旋体的60千道尔顿CA。编码伯氏疏螺旋体CA的DNA区域位于质粒pKH1的2.3千碱基片段上。CA可能在莱姆病螺旋体病中具有致病意义，因为最近已表明分枝杆菌的CA在实验性自身免疫性关节炎的病因中起作用。这种抗原的广泛交叉反应性可能解释了目前用于莱姆病螺旋体病血清学检测的低诊断特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/1e33b132c054/iai00080-0232-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/5f1f6bcc5268/iai00080-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/9785e45b50d9/iai00080-0231-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/07d020bbb8b2/iai00080-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/1e33b132c054/iai00080-0232-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/5f1f6bcc5268/iai00080-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/9785e45b50d9/iai00080-0231-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/07d020bbb8b2/iai00080-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2b8/259521/1e33b132c054/iai00080-0232-b.jpg

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