利用 X 射线自由电子激光实时研究腺嘌呤核糖开关。
Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser.
机构信息
Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD, USA.
出版信息
FEBS J. 2017 Oct;284(20):3374-3380. doi: 10.1111/febs.14110. Epub 2017 Aug 1.
Abstract
Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecular 'switch'.
摘要
通过连续的飞秒晶体学，解析了腺嘌呤核糖开关适体结构域的四个反应状态的结构，包括一个瞬时中间状态。这些结构不仅证明了 X 射线自由电子激光在 RNA 晶体学中的应用，还证明了通过混合注入晶体学可以实时确定瞬态状态。这些结果说明了与分子“开关”相关的配体诱导构象变化的结构基础。
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