Nakamura Y, Lathrop M, O'Connell P, Leppert M, Barker D, Wright E, Skolnick M, Kondoleon S, Litt M, Lalouel J M
Howard Hughes Medical Institute, University of Utah Health Sciences Center, Salt Lake City 84132.
Genomics. 1988 May;2(4):302-9. doi: 10.1016/0888-7543(88)90018-3.
We have developed and mapped by genetic linkage a primary set of markers for chromosome 17. The map consists of 21 loci derived from 27 probe/enzyme systems, including eight highly informative markers at loci containing a variable number of tandemly repeated DNA sequences (VNTRs). The map is continuous from the telomeric region of the short arm to the telomeric region of the long arm, covering estimated genetic distances of 218 cM in males and 279 cM in females. The average heterozygosity among all 21 loci in the population sample analyzed is 58%; 77% heterozygosity was observed among the eight VNTR markers that were highly informative. This map will make it possible to detect by linkage the location of genetic defects associated with chromosome 17 and will also provide anchor points for a high-resolution map of this chromosome.
我们通过遗传连锁分析开发并绘制了17号染色体的一组主要标记。该图谱由27个探针/酶系统衍生出的21个位点组成，包括8个位于含有可变数量串联重复DNA序列（VNTRs）位点的高信息含量标记。该图谱从短臂的端粒区域到长臂的端粒区域是连续的，在男性中覆盖的估计遗传距离为218厘摩，在女性中为279厘摩。在所分析的群体样本中，所有21个位点的平均杂合度为58%；在8个高信息含量的VNTR标记中观察到77%的杂合度。该图谱将使得通过连锁分析检测与17号染色体相关的遗传缺陷的位置成为可能，并且还将为该染色体的高分辨率图谱提供锚定标记。
