Yamauchi R, Itabashi T, Wada K, Tanaka T, Kumagai G, Ishibashi Y
Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562 Aomori, Japan
Department of Surgery, Kuroishi General Hospital, 1 Chome-70 Kitamicho, Kuroishi, Aomori Prefecture 036-0541, Japan.
Bone Joint Res. 2017 May;6(5):331-336. doi: 10.1302/2046-3758.65.BJR-2016-0221.R1.
Ultraviolet (UV) light-mediated photofunctionalisation is known to improve osseointegration of pure titanium (Ti). However, histological examination of titanium alloy (Ti6Al4V), which is frequently applied in orthopaedic and dental surgery, has not yet been performed. This study examined the osseointegration of photofunctionalised Ti6Al4V implants.
Ti and Ti6Al4V implants were treated with UV light, and the chemical composition and contact angle on the surfaces were evaluated to confirm photofunctionalisation. The implants were inserted into femurs in rats, and the rats were killed two or four weeks after the surgery. For histomorphometric analysis, both the bone-implant contact (BIC) ratio and the bone volume (BV) ratio were calculated from histological analysis and microcomputed tomography data.
The amount of carbon and the contact angle on both implants were significantly reduced after UV irradiation. The BIC ratios for both UV light-treated implants significantly increased at two weeks, but there was no significant difference at four weeks. There was no significant difference in the BV ratios between the UV light-treated and control implants at two or four weeks.
This study suggests that photofunctionalisation of Ti6Al4V implants, similar to that of Ti implants, may promotes osseointegration in early but not in the late phase of osseointegration. R. Yamauchi, T. Itabashi, K. Wada, T. Tanaka, G. Kumagai, Y. Ishibashi. Photofunctionalised Ti6Al4V implants enhance early phase osseointegration. 2017;6:331-336. DOI: 10.1302/2046-3758.65.BJR-2016-0221.R1.
已知紫外线（UV）介导的光功能化可改善纯钛（Ti）的骨整合。然而，常用于骨科和牙科手术的钛合金（Ti6Al4V）的组织学检查尚未开展。本研究检测了光功能化Ti6Al4V植入物的骨整合情况。
对Ti和Ti6Al4V植入物进行紫外线处理，并评估其表面的化学成分和接触角以确认光功能化。将植入物植入大鼠股骨，术后两周或四周处死大鼠。对于组织形态计量学分析，根据组织学分析和微型计算机断层扫描数据计算骨-植入物接触（BIC）率和骨体积（BV）率。
紫外线照射后，两种植入物的碳含量和接触角均显著降低。两种经紫外线处理的植入物的BIC率在两周时均显著增加，但在四周时无显著差异。在两周或四周时，经紫外线处理的植入物与对照植入物的BV率无显著差异。
本研究表明，Ti6Al4V植入物的光功能化与Ti植入物类似，可能在骨整合的早期而非晚期促进骨整合。R. Yamauchi、T. Itabashi、K. Wada、T. Tanaka、G. Kumagai、Y. Ishibashi。光功能化Ti6Al4V植入物增强早期骨整合。2017；6：331 - 336。DOI：10.1302/2046 - 3758.65.BJR - 2016 - 0221.R1。
