Julák J, Soušková H, Scholtz V, Kvasničková E, Savická D, Kříha V
Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, 128 00, Prague, Czech Republic.
Department of Computing and Control Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, 166 28, Prague, Czech Republic.
Folia Microbiol (Praha). 2018 Jan;63(1):63-68. doi: 10.1007/s12223-017-0535-6. Epub 2017 Jun 17.
The inactivation of four micromycete species by action of non-thermal plasma was followed. Two sources of plasma were compared, namely, positive corona discharge and dielectric barrier discharge. The corona discharge appeared as suitable for fungal spore inactivation in water suspension, whereas the barrier discharge inactivated spores on the surface of cultivation agar. Cladosporium sphaerospermum was the most sensitive, being inactivated within 10 min of exposure to plasma, whereas Aspergillus oryzae displayed decrease in viable cell count only, the complete inactivation was not achieved even after 40 min of exposure. Intermediate sensitivity was found for Alternaria sp. and Byssochlamys nivea. The significant delay of growth was observed for all fungi after exposure to sublethal dose of plasma, but we failed to express this effect quantitatively.
研究了非热等离子体作用对四种微真菌物种的灭活情况。比较了两种等离子体源，即正电晕放电和介质阻挡放电。电晕放电似乎适用于水悬浮液中真菌孢子的灭活，而介质阻挡放电可使培养琼脂表面的孢子失活。球形枝孢菌最为敏感，暴露于等离子体10分钟内即被灭活，而米曲霉仅活细胞数减少，即使暴露40分钟后也未实现完全灭活。链格孢属和雪白丝衣霉表现出中等敏感性。所有真菌在暴露于亚致死剂量的等离子体后均观察到生长显著延迟，但我们未能对这种效应进行定量表达。
