Falahati M, Sepahvand A, Mahmoudvand H, Baharvand P, Jabbarnia S, Ghojoghi A, Yarahmadi M
Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Curr Med Mycol. 2015 Sep;1(3):25-32. doi: 10.18869/acadpub.cmm.1.3.25.
Despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. The present study was designed to investigate the antifungal properties of different extracts from Desf.
Different parts of (i.e., dried fruit, fresh fruit and dried leaf) were separately extracted via percolation method with 80% methanol and water. Gas chromatography/mass spectrometry (GC/MS) analysis was performed to determine the main constituents of leaf and fruit extracts from . anti- activities of the extracts against , and were studied. For this purpose, the minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) were determined, using broth microdilution method, according to the modified M27-A3 protocol on yeasts, proposed by the Clinical and Laboratory Standards Institute (CLSI).
Based on GC/MS analysis, the main constituents of fruit extracts were β-myrcene (41.4%), α-pinene (32.48%) and limonene (4.66%), respectively, whereas the major constituents of leaf extracts were trans-caryophyllene (15.18%), α-amorphene (8.1%) and neo-allo-ocimene (6.21%), respectively. As the findings indicated, all the constituents exhibited both fungistatic and fungicidal activities, with MICs ranging from 6.66 to 26.66 mg/mL and MFCs ranging from 13.3 to 37.3 mg/mL, respectively. Among the evaluated extracts, the methanolic fresh fruit extract of was significantly more effective than other extracts (P<0.05).
Based on the findings of the present study, novel antifungal agents need to be developed, and use of should be promoted in the traditional treatment of infections.
尽管有多种治疗真菌疾病的方法，但由于多种与治疗相关的副作用，这些疾病的管理仍存在一些局限性。本研究旨在调查Desf.不同提取物的抗真菌特性。
通过渗漉法分别用80%甲醇和水提取Desf.的不同部位（即干果、鲜果和干叶）。采用气相色谱/质谱（GC/MS）分析确定Desf.叶和果实提取物的主要成分。研究了提取物对白色念珠菌、热带念珠菌和近平滑念珠菌的抗真菌活性。为此，根据临床和实验室标准协会（CLSI）提出的关于酵母的改良M27 - A3方案，采用肉汤微量稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。
基于GC/MS分析，Desf.果实提取物的主要成分分别为β-月桂烯（41.4%）、α-蒎烯（32.48%）和柠檬烯（4.66%），而Desf.叶提取物的主要成分分别为反式石竹烯（15.18%）、α-紫穗槐烯（8.1%）和新别罗勒烯（6.21%）。研究结果表明，所有成分均表现出抑菌和杀菌活性，MIC范围为6.66至26.66 mg/mL，MFC范围为13.3至37.3 mg/mL。在所评估的提取物中，Desf.的甲醇鲜果提取物比其他提取物显著更有效（P<0.05）。
基于本研究结果，需要开发新型抗真菌药物，并应在传统治疗念珠菌感染中推广使用Desf.。
