State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
Laboratory Department, Navy General Hospital, Beijing, 100048, China.
Sci Rep. 2017 Jul 19;7(1):5830. doi: 10.1038/s41598-017-06283-z.
This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones of p0716-KPC/p12181-KPC contained two different replicons (belonging to a novel IncFII subtype and the Rep_3 family), the IncFII and IncFII maintenance regions, and conjugal transfer gene sets from IncFII-type plasmids and unknown origins. p0716-KPC and p12181-KPC carried similar three accessory resistance regions, namely ΔTn6209, a MDR region, and the bla region. Resistance genes bla , mph(A), strAB, aacC2, qacEΔ1, sul1, sul2, and dfrA25, which are associated with transposons, integrons, and insertion sequence-based mobile units, were located in these accessory regions. p0716-KPC carried two additional resistance genes: aphA1a and bla . Together, our analyses showed that p0716-KPC and p12181-KPC belong to a novel IncFII subtype and display a complex chimeric nature, and that the carbapenem resistance gene bla coexists with a lot of additional resistance genes on these two plasmids.
本研究旨在对从两个不同临床分离的肺炎克雷伯菌中回收的两个完全测序的新型 IncFII 型多药耐药（MDR）质粒 p0716-KPC 和 p12181-KPC 进行基因特征分析。p0716-KPC 和 p12181-KPC 具有非常相似的基因组内容。p0716-KPC/p12181-KPC 的骨架包含两个不同的复制子（属于新型 IncFII 亚型和 Rep_3 家族）、IncFII 和 IncFII 维持区，以及来自 IncFII 型质粒和未知来源的接合转移基因簇。p0716-KPC 和 p12181-KPC 携带相似的三个辅助耐药区，即ΔTn6209、MDR 区和 bla 区。bla 、mph(A)、strAB、aacC2、qacEΔ1、sul1、sul2 和 dfrA25 等耐药基因与转座子、整合子和基于插入序列的移动单元有关，位于这些辅助区。p0716-KPC 携带另外两个耐药基因：aphA1a 和 bla 。综上所述，我们的分析表明，p0716-KPC 和 p12181-KPC 属于新型 IncFII 亚型，显示出复杂的嵌合性质，而碳青霉烯耐药基因 bla 与这两个质粒上的许多其他耐药基因共存。
