Wang Yujuan, Xia Han, Zhang Bo, Liu Xiaoyun, Yuan Zhiming
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Virus Res. 2017 Aug 15;240:130-139. doi: 10.1016/j.virusres.2017.08.001. Epub 2017 Aug 18.
A new insect nidovirus (named Yichang virus) from the family Mesoniviridae was isolated, identified, and characterized from Culex mosquitoes in Hubei, China. Results showed a high number of viral RNA copies (up to 10 copies/ml) within 48h in C6/36 cells. In addition, the titers of the Yichang virus reached maximal levels of 10 PFU/mL at 6 d post-infection (dpi). The virus produced moderate cytopathic effects when the multiplicity of infection ranged from 0.001-0.1 at 6 dpi, but did not replicate in mammalian cells. Under electron microscopy, the virion of the Yichang virus appeared as spherical particles with diameters of ∼80nm and large club-shaped projections. Although subsequent genomic sequence analysis revealed that the Yichang virus had similar protein patterns as those of other mesoniviruses, the nucleotide acids shared less than 20% BLAST query coverage with known viruses in the family Mesoniviridae, and showed a maximum sequence identity of 67% for RNA-dependent RNA polymerase (RdRp). The putative protein sequences showed slightly higher identity (28%-68%), and the most conserved domain was RdRp. Based on the phylogenetic and pairwise evolutionary distance analyses, the Yichang virus should be considered a new species belonging to a currently unassigned genus within the family Mesoniviridae.
从中国湖北的库蚊中分离、鉴定并表征了一种来自中传病毒科的新型昆虫正呼肠孤病毒（命名为宜昌病毒）。结果显示，在C6/36细胞中，48小时内病毒RNA拷贝数很高（高达10拷贝/毫升）。此外，宜昌病毒的滴度在感染后6天（dpi）达到最高水平10 PFU/mL。当感染复数在6 dpi时为0.001 - 0.1时，该病毒产生中度细胞病变效应，但在哺乳动物细胞中不复制。在电子显微镜下，宜昌病毒的病毒粒子呈直径约80nm的球形颗粒，并带有大的棒状突起。尽管随后的基因组序列分析表明，宜昌病毒与其他中传病毒具有相似的蛋白质模式，但与中传病毒科已知病毒的核苷酸BLAST查询覆盖率低于20%，并且其RNA依赖的RNA聚合酶（RdRp）的最大序列同一性为67%。推测的蛋白质序列显示出略高的同一性（28% - 68%），最保守的结构域是RdRp。基于系统发育和成对进化距离分析，宜昌病毒应被视为中传病毒科内一个目前未分类属的新物种。
