聚甲基丙烯酸甲酯/聚乙烯醇共聚物剂应用于糖尿病伤口敷料。
Poly-Methyl Methacrylate/Polyvinyl Alcohol Copolymer Agents Applied on Diabetic Wound Dressing.
机构信息
Graduate Institute of Medical Science, College of Medicine, Taipei Medical University, No. 250 Wuxing St., Taipei, 11031, Taiwan.
Department of Anatomy and Cell Biology, School of Medicine, Taipei Medical University, No. 250 Wuxing St., Taipei, 11031, Taiwan.
出版信息
Sci Rep. 2017 Aug 25;7(1):9531. doi: 10.1038/s41598-017-10193-5.
Due to the difficulty of healing chronic wound, in the process of changing dressing, secondary damage on the tissue caused by adhesion should be prevented. In this study, the new dressing of particle hydrogels synthesized with poly-methyl methacrylate and poly-vinyl alcohol precursors were proposed. In addition, cell safety tests, animal's allergic stimulation, and animal's wound healing experiments were conducted for particle hydrogels. On one hand, in L929 cell experiment, the results of particle hydrogels extract 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide tests and lactate dehydrogenase test trial show that there are no safety concerns over particle hydrogels. On the other hand, New Zealand white rabbits were chosen for skin sensitization tests in animal trials, which show the consistent results. At last, wound healing tests used diabetes induction with 10-week-old rats and three-month-old Landrace pigs, with the tissue histology. In short, through this experiment, it is found that in the early phase of the diabetic rats and pigs' wound healing, using particle hydrogels can enhance collagen formation, and achieve the goal of faster wound healing.
由于慢性伤口难以愈合，在换药过程中应防止粘连对组织造成的二次损伤。本研究提出了一种由聚甲基丙烯酸甲酯和聚乙烯醇前体合成的新型颗粒水凝胶敷料。此外，还对颗粒水凝胶进行了细胞安全性试验、动物过敏刺激试验和动物伤口愈合试验。一方面，在 L929 细胞实验中，颗粒水凝胶提取液 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐试验和乳酸脱氢酶试验结果表明，颗粒水凝胶不存在安全问题。另一方面，在动物试验中，选用新西兰白兔进行皮肤致敏试验，结果一致。最后，采用 10 周龄大鼠和 3 月龄长白猪诱导糖尿病进行伤口愈合试验，并进行组织学检查。总之，通过该实验发现，在糖尿病大鼠和猪伤口愈合的早期阶段，使用颗粒水凝胶可以促进胶原蛋白的形成，从而达到更快愈合的目的。
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