Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
J Biomed Sci. 2010 Aug 24;17 Suppl 1(Suppl 1):S31. doi: 10.1186/1423-0127-17-S1-S31.
Perinatal taurine exposure influences renal function in adult female offspring. This study tests the hypothesis that prenatal rather than postnatal taurine exposure alters renal function in adult conscious male rats. Female Sprague Dawley rats were fed normal rat chow and tap water alone (Control), tap water containing 3% beta-alanine (taurine depletion, TD) or tap water containing 3% taurine (taurine supplementation, TS) either from conception until delivery (fetal period; TDF or TSF) or from delivery until weaning (lactation period; TDL or TSL). After weaning, male offspring were fed with the normal rat chow and tap water ad libitum. At 7-8 weeks of age, renal function was studied in conscious, restrained rats. Mean arterial pressures were slightly higher in rats receiving taurine supplementation during either the fetal or lactation periods (compared to Control and TD groups), but heart rates were not significantly different among groups. Effective renal blood flows were lower in TDF, TDL, and TSF rats (TDF 4.6+/-0.8 ml/min/g kidney weight (KW), TDL 3.0+/-0.9 ml/min/g KW, and TSF 2.8+/-0.7 ml/min/g KW) than in TSL (7.7+/-0.9 ml/min/g KW) or Control rats (7.3+/-1.6 ml/min/g KW). These differences were correlated with significant increases in renal vascular resistance in TDF, TDL, and TSF groups compared to TSL and Control rats. In contrast, glomerular filtration rates were not significantly different among groups. Although basal water and sodium excretion were slightly lower in TDL and TSF rats compared to other groups, their diuretic and natriuretic responses to an acute saline load were not different from Control. The present data indicate that in adult male rats, both perinatal supplementation and depletion of taurine can alter renal hemodynamics, and these effects are differentially time-dependent.
围产期牛磺酸暴露会影响成年雌性后代的肾功能。本研究检验了这样一个假设，即产前而非产后牛磺酸暴露会改变成年雄性大鼠的肾功能。雌性 Sprague Dawley 大鼠喂食普通大鼠饲料和自来水（对照组）、含 3%β-丙氨酸（牛磺酸耗竭，TD）的自来水或含 3%牛磺酸（牛磺酸补充，TS）的自来水，从受孕到分娩（胎儿期；TDF 或 TSF）或从分娩到断奶（哺乳期；TDL 或 TSL）。断奶后，雄性幼鼠自由喂食普通大鼠饲料和自来水。在 7-8 周龄时，对清醒、束缚的大鼠进行肾功能研究。与对照组和 TD 组相比，在胎儿期或哺乳期接受牛磺酸补充的大鼠的平均动脉压略高，但心率在各组间无显著差异。TDF、TDL 和 TSF 组的有效肾血流量较低（TDF 为 4.6+/-0.8 ml/min/g 肾重（KW），TDL 为 3.0+/-0.9 ml/min/g KW，TSF 为 2.8+/-0.7 ml/min/g KW），低于 TSL（7.7+/-0.9 ml/min/g KW）或对照组大鼠（7.3+/-1.6 ml/min/g KW）。这些差异与 TDF、TDL 和 TSF 组的肾血管阻力显著增加有关，与 TSL 和对照组大鼠相比。相比之下，各组之间肾小球滤过率没有显著差异。尽管 TDL 和 TSF 组大鼠的基础水和钠排泄量略低于其他组，但它们对急性盐水负荷的利尿和排钠反应与对照组无差异。本研究数据表明，在成年雄性大鼠中，围产期牛磺酸的补充和耗竭均可改变肾功能的血液动力学，且这些影响具有不同的时间依赖性。
