Yassin Afrah Kamal, Gong Jiansen, Kelly Patrick, Lu Guangwu, Guardabassi Luca, Wei Lanjing, Han Xiangan, Qiu Haixiang, Price Stuart, Cheng Darong, Wang Chengming
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu, PR China.
Department of Food Hygiene and safety, Faculty of Public and Environmental Health, Khartoum University, Khartoum, Sudan.
PLoS One. 2017 Sep 21;12(9):e0185326. doi: 10.1371/journal.pone.0185326. eCollection 2017.
Poultry and livestock are the most important reservoirs for pathogenic Escherichia coli and use of antimicrobials in animal farming is considered the most important factor promoting the emergence, selection and dissemination of antimicrobial-resistant microorganisms. The aim of our study was to investigate antimicrobial resistance in E. coli isolated from food animals in Jiangsu, China. The disc diffusion method was used to determine susceptibility to 18 antimicrobial agents in 862 clinical isolates collected from chickens, ducks, pigs, and cows between 2004 and 2012. Overall, 94% of the isolates showed resistance to at least one drug with 83% being resistance to at least three different classes of antimicrobials. The isolates from the different species were most commonly resistant to tetracycline, nalidixic acid, sulfamethoxazole, trimethoprim/sulfamethoxazole and ampicillin, and showed increasing resistance to amikacin, aztreonam, ceftazidime, cefotaxime, chloramphenicol, ciprofloxacin. They were least resistant to amoxicillin/clavulanic acid (3.4%) and ertapenem (0.2%). MDR was most common in isolates from ducks (44/44, 100%), followed by chickens (568/644, 88.2%), pigs (93/113, 82.3%) and cows (13/61, 21.3%). Our finding that clinical E. coli isolates from poultry and livestock are commonly resistant to multiple antibiotics should alert public health and veterinary authorities to limit and rationalize antimicrobial use in China.
家禽和家畜是致病性大肠杆菌最重要的宿主，在动物养殖中使用抗菌药物被认为是促进耐药微生物出现、选择和传播的最重要因素。我们研究的目的是调查从中国江苏的食用动物中分离出的大肠杆菌的耐药性。采用纸片扩散法测定了2004年至2012年间从鸡、鸭、猪和牛中收集的862株临床分离株对18种抗菌药物的敏感性。总体而言，94%的分离株对至少一种药物耐药，83%的分离株对至少三类不同抗菌药物耐药。不同物种的分离株最常对四环素、萘啶酸、磺胺甲恶唑、甲氧苄啶/磺胺甲恶唑和氨苄西林耐药，并且对阿米卡星、氨曲南、头孢他啶、头孢噻肟、氯霉素、环丙沙星的耐药性呈上升趋势。它们对阿莫西林/克拉维酸（3.4%）和厄他培南（0.2%）的耐药性最低。多重耐药在鸭的分离株中最为常见（44/44，100%），其次是鸡（568/644，88.2%）、猪（93/113，82.3%）和牛（13/61，21.3%）。我们的研究发现，来自家禽和家畜的临床大肠杆菌分离株通常对多种抗生素耐药，这应提醒公共卫生和兽医当局限制并合理使用中国的抗菌药物。
